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ilvC ketol-acid reductoisomerase (NADP(+)) [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948286, updated on 11-Apr-2024

Summary

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Gene symbol
ilvC
Gene description
ketol-acid reductoisomerase (NADP(+))
Primary source
ASAP:ABE-0012331
Locus tag
b3774
See related
ECOCYC:EG10495
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3766
Summary
Acetohydroxy acid isomeroreductase (IlvC) carries out two analogous keto-acid reductoisomerase reactions in the parallel biosynthetic pathways generating isoleucine and valine. [More information is available at EcoCyc: EG10495].
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Genomic context

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Sequence:
NC_000913.3 (3957970..3959445)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene threonine deaminase Neighboring gene DNA-binding transcriptional dual regulator IlvY Neighboring gene peptidyl-prolyl cis-trans isomerase C Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Metal Ions Play an Essential Catalytic Role in the Mechanism of Ketol-Acid Reductoisomerase. Tadrowski S, et al. Chemistry, 2016 May 23. PMID 27136273
  2. General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH. Brinkmann-Chen S, et al. Proc Natl Acad Sci U S A, 2013 Jul 2. PMID 23776225, Free PMC Article
  3. Bacterial and plant ketol-acid reductoisomerases have different mechanisms of induced fit during the catalytic cycle. Wong SH, et al. J Mol Biol, 2012 Dec 7. PMID 23036858
  4. Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli. Bastian S, et al. Metab Eng, 2011 May. PMID 21515217
  5. The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution. Tyagi R, et al. Protein Sci, 2005 Dec. PMID 16322583, Free PMC Article

See all (45) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Key cofactor specificity determinants are identified and five enzymes constructed with reversed cofactor preference; additional directed evolution generated two enzymes with NADH-dependent catalytic efficiencies greater than wild-type enzymes with NADPH.
    Title: General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH.
  2. Ultracentrifugation studies confirm that the enzyme exists as a tetramer in solution, and isothermal titration calorimetry shows that the binding of Mg(2+) decreases, while the binding of NADPH increases the structural rigidity of the enzyme.
    Title: Bacterial and plant ketol-acid reductoisomerases have different mechanisms of induced fit during the catalytic cycle.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
  4. crystal structure of Escherichia coli ketol-acid reductoisomerase
    Title: Facile crystallization of Escherichia coli ketol-acid reductoisomerase.
  5. crystal structure of the E. coli enzyme at 2.6 A resolution, the first three-dimensional structure of any bacterial Class II KARI
    Title: The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2-dehydropantoate 2-reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NADP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ketol-acid reductoisomerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ketol-acid reductoisomerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ketol-acid reductoisomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in branched-chain amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in isoleucine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in isoleucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within isoleucine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within pantothenate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in valine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in valine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within valine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ketol-acid reductoisomerase (NADP(+))
NP_418222.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3957970..3959445
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418222.1 ketol-acid reductoisomerase (NADP(+)) [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418222.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ARA2, A0A8S7JHE0
    Conserved Domains (1) summary
    PRK05225
    Location:2 → 488
    PRK05225; ketol-acid reductoisomerase; Validated
Nucleotide Protein
Heading Accession and Version

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