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pldA outer membrane phospholipase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948307, updated on 11-Apr-2024

Summary

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Gene symbol
pldA
Gene description
outer membrane phospholipase A
Primary source
ASAP:ABE-0012479
Locus tag
b3821
See related
ECOCYC:EG10738
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3815
Summary
PldA is the Phospholipase A1 precursor which is a member of EC 3.1.1.32. [More information is available at EcoCyc: EG10738].
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Genomic context

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Sequence:
NC_000913.3 (4004862..4005731)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter RarD Neighboring gene putative thioesterase YigI Neighboring gene ATP-dependent DNA helicase RecQ Neighboring gene L-threonine exporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy. Rangl M, et al. J Mol Biol, 2017 Apr 7. PMID 28283404
  2. Outer membrane phospholipase a dimer stability does not correlate to occluded surface area. Ebie Tan A, et al. Biochemistry, 2008 Nov 18. PMID 18939857
  3. The role of a hydrogen bonding network in the transmembrane beta-barrel OMPLA. Stanley AM, et al. J Mol Biol, 2007 Jul 27. PMID 17555765
  4. Energetics of outer membrane phospholipase A (OMPLA) dimerization. Stanley AM, et al. J Mol Biol, 2006 Apr 21. PMID 16497324
  5. Assembly of colicin A in the outer membrane of producing Escherichia coli cells requires both phospholipase A and one porin, but phospholipase A is sufficient for secretion. Cavard D. J Bacteriol, 2002 Jul. PMID 12057969, Free PMC Article

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Upon the addition of Ca(2+), OmpLA was activated and degraded the membrane with a turnover of ~2 phospholipid molecules per second and per OmpLA dimer until most of the membrane phospholipids were hydrolyzed and the protein became tightly packed.
    Title: Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy.
  2. The crystal structure of the dimeric state of the transmembrane beta-barrel protein outer membrane phospholipase A (OMPLA) revealed an intermolecular hydrogen bond mediated by a highly conserved glutamine side-chain (Q94).
    Title: The role of a hydrogen bonding network in the transmembrane beta-barrel OMPLA.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli.
  4. These energetic measurements not only provide insight into the activation of OMPLA, but they also represent the first quantitative investigation of the association energetics of a transmembrane beta-barrel.
    Title: Energetics of outer membrane phospholipase A (OMPLA) dimerization.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 1-acyl-2-lysophosphatidylserine acylhydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables calcium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables lysophospholipase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylserine 1-acylhydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables phospholipase A1 activity IEA
Inferred from Electronic Annotation
more info
  
enables phospholipase A1 activity TAS
Traceable Author Statement
more info
 PubMed 
enables phospholipase A2 activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phospholipase A2 activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipase A2 activity IEA
Inferred from Electronic Annotation
more info
  
enables phospholipase A2 activity TAS
Traceable Author Statement
more info
 PubMed 
enables phospholipase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in lipid catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphatidylglycerol metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
outer membrane phospholipase A
NP_418265.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4004862..4005731
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418265.1 outer membrane phospholipase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418265.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    C3SK99
    Conserved Domains (1) summary
    PRK10763
    Location:1 → 289
    PRK10763; phospholipase A; Provisional
Nucleotide Protein
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