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metF 5,10-methylenetetrahydrofolate reductase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948432, updated on 25-Apr-2024

Summary

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Gene symbol
metF
Gene description
5,10-methylenetetrahydrofolate reductase
Primary source
ASAP:ABE-0012897
Locus tag
b3941
See related
ECOCYC:EG10585
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3933
Summary
Upregulated by GSNO in defined medium. [More information is available at EcoGene: EG10585].
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Genomic context

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Sequence:
NC_000913.3 (4132616..4133506)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene O-succinylhomoserine(thiol)-lyase/O-succinylhomoserine lyase Neighboring gene fused aspartate kinase/homoserine dehydrogenase 2 Neighboring gene catalase/hydroperoxidase HPI Neighboring gene cystine exporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural perturbations in the Ala --> Val polymorphism of methylenetetrahydrofolate reductase: how binding of folates may protect against inactivation. Pejchal R, et al. Biochemistry, 2006 Apr 18. PMID 16605249, Free PMC Article
  2. Structures of NADH and CH3-H4folate complexes of Escherichia coli methylenetetrahydrofolate reductase reveal a spartan strategy for a ping-pong reaction. Pejchal R, et al. Biochemistry, 2005 Aug 30. PMID 16114881
  3. Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: roles for aspartate 120 and glutamate 28. Trimmer EE, et al. Biochemistry, 2001 May 29. PMID 11371182
  4. Methylenetetrahydrofolate reductase from Escherichia coli: elucidation of the kinetic mechanism by steady-state and rapid-reaction studies. Trimmer EE, et al. Biochemistry, 2001 May 29. PMID 11371181
  5. Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. Sheppard CA, et al. J Bacteriol, 1999 Feb. PMID 9922232, Free PMC Article

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results suggest that Leu may be a suitable replacement for Phe223 in the oxidative half-reaction of E. coli methylenetetrahydrofolate reductase.
    Title: Functional role for the conformationally mobile phenylalanine 223 in the reaction of methylenetetrahydrofolate reductase from Escherichia coli.
  2. The invariant aspartate 120 is a critical residue for E. coli methylenetetrahydrofolate reductase, with major roles in folate binding and catalysis, and a minor role in redox modulation.
    Title: Aspartate 120 of Escherichia coli methylenetetrahydrofolate reductase: evidence for major roles in folate binding and catalysis and a minor role in flavin reactivity.
  3. Multiple onformation, which allows aspartic acid 120 to hydrogen bond to the pterin ring in the folate complex but must move to an "open" conformation to allow NADH to bind.
    Title: Structures of NADH and CH3-H4folate complexes of Escherichia coli methylenetetrahydrofolate reductase reveal a spartan strategy for a ping-pong reaction.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables FAD binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables FAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylenetetrahydrofolate reductase (NAD(P)H) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylenetetrahydrofolate reductase (NAD(P)H) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylenetetrahydrofolate reductase (NAD(P)H) activity IEA
Inferred from Electronic Annotation
more info
  
enables methylenetetrahydrofolate reductase (NADH) activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein-folding chaperone binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in methionine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in methionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in methionine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tetrahydrofolate interconversion IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
5,10-methylenetetrahydrofolate reductase
NP_418376.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4132616..4133506
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418376.1 5,10-methylenetetrahydrofolate reductase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418376.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    F4V8T8
    Conserved Domains (1) summary
    PRK09432
    Location:1 → 296
    metF; methylenetetrahydrofolate reductase
Nucleotide Protein
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