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btuB cobalamin outer membrane transporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948468, updated on 11-Apr-2024

Summary

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Gene symbol
btuB
Gene description
cobalamin outer membrane transporter
Primary source
ASAP:ABE-0012987
Locus tag
b3966
See related
ECOCYC:EG10126
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
bfe; cer; ECK3958
Summary
BtuB translation is regulated by adenosylcobalamin binding directly to btuB mRNA, a B12-responsive riboswitch. [More information is available at EcoGene: EG10126]. BtuB is an outer membrane porin that mediates high affinity binding and TonB- dependent active transport of vitamin B12 (cyanocobalamin) across the outer membrane. [More information is available at EcoCyc: EG10126].
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Genomic context

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Sequence:
NC_000913.3 (4163639..4165483)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor FabR Neighboring gene DUF1422 domain-containing inner membrane protein YijD Neighboring gene tRNA m(5)U54 methyltransferase Neighboring gene glutamate racemase Neighboring gene 16S ribosomal RNA Neighboring gene tRNA-Glu

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A kissing loop is important for btuB riboswitch ligand sensing and regulatory control. Lussier A, et al. J Biol Chem, 2015 Oct 30. PMID 26370077, Free PMC Article
  2. Mg(2+)-induced conformational changes in the btuB riboswitch from E. coli. Choudhary PK, et al. RNA, 2014 Jan. PMID 24243114, Free PMC Article
  3. The N-terminal domain of a TonB-dependent transporter undergoes a reversible stepwise denaturation. Flores Jiménez RH, et al. Biochemistry, 2012 May 1. PMID 22497281, Free PMC Article
  4. Molecular origin of electron paramagnetic resonance line shapes on β-barrel membrane proteins: the local solvation environment modulates spin-label configuration. Freed DM, et al. Biochemistry, 2011 Oct 18. PMID 21894979, Free PMC Article
  5. The effect of calcium on the conformation of cobalamin transporter BtuB. Luan B, et al. Proteins, 2010 Apr. PMID 19927326, Free PMC Article

See all (88) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
    Title: HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
  2. The btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression.
    Title: A kissing loop is important for btuB riboswitch ligand sensing and regulatory control.
  3. The lack of a AdoCbl-induced conformational switch experienced by the btuB riboswitch in the absence of Mg(2+) indicates a crucial role played by Mg(2+) for defining an active conformation of the riboswitch.
    Title: Mg(2+)-induced conformational changes in the btuB riboswitch from E. coli.
  4. Through biological and biochemical analysis, authors have demonstrated the GadX can directly interact with btuB promoter and affect the expression of btuB.
    Title: Repression of btuB gene transcription in Escherichia coli by the GadX protein.
  5. A portion of the N-terminal region of BtuB which occupies the lumen of the BtuB barrel denatures prior to the unfolding of the BtuB barrel, so that the free energy of folding for the N-terminus is smaller than that typically seen for globular proteins.
    Title: The N-terminal domain of a TonB-dependent transporter undergoes a reversible stepwise denaturation.
  6. analysis of the conformation of BtuB by electron paramagnetic resonance
    Title: Molecular origin of electron paramagnetic resonance line shapes on β-barrel membrane proteins: the local solvation environment modulates spin-label configuration.
  7. Data describe the effect of calcium ions on the conformation of the extracellular loops of BtuB and their possible role in B12 binding.
    Title: The effect of calcium on the conformation of cobalamin transporter BtuB.
  8. High concentrations of stabilizing solutes, such as those used to generate membrane protein crystals, result in a more compact and ordered state of the protein than is seen under more physiological conditions.
    Title: Solutes alter the conformation of the ligand binding loops in outer membrane transporters.
  9. the role of the TonB-ExbB-ExbD complex, potentiated by the proton motive force, is to reduce the affinity of the Cbl-binding site, thus increasing the rate of Cbl release into the periplasmic space
    Title: Observations on the calcium dependence and reversibility of cobalamin transport across the outer membrane of Escherichia coli.
  10. The data indicate that charged residues (D316 and R14) are critical to the unfolding and conformational equilibrium of the energy-coupling motif.
    Title: Molecular basis for substrate-dependent transmembrane signaling in an outer-membrane transporter.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ABC-type vitamin B12 transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables porin activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein domain specific binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables vitamin transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cobalamin transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cobalamin transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cobalamin transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cobalamin transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cobalamin transport NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of monoatomic ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in vitamin transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cell outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of pore complex IEA
Inferred from Electronic Annotation
more info
  
part_of transmembrane transporter complex NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
cobalamin outer membrane transporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4163639..4165483
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418401.1 cobalamin outer membrane transporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418401.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7ELV7
    Conserved Domains (1) summary
    PRK10641
    Location:1 → 614
    btuB; TonB-dependent vitamin B12 receptor BtuB
Nucleotide Protein
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Other Literature Sources
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Research Materials