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thiC phosphomethylpyrimidine synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948492, updated on 4-Apr-2024

Summary

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Gene symbol
thiC
Gene description
phosphomethylpyrimidine synthase
Primary source
ASAP:ABE-0013057
Locus tag
b3994
See related
ECOCYC:EG11585
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3986
Summary
In vitro Salmonella ThiC produces HMP (4-amino-2-methyl-5-hydroxymethylpyrimidine) from AIR (5-amino-1-(5-phospho-D-ribosyl)imidazole) utilizing SAM and an iron-sulfur cluster in a reaction reminiscent of Radical SAM family reactions although ThiC does not contain the Radical SAM Cys signature motif (Martinez-Gomez, 2008). [More information is available at EcoGene: EG11585]. The gene has been sequenced. [More information is available at EcoCyc: EG11585].
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Genomic context

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Sequence:
NC_000913.3 (4194204..4196099, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene sulfur carrier protein ThiS Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene thiamine phosphate synthase Neighboring gene regulator of sigma D Neighboring gene RNA decapping hydrolase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation. Chauvier A, et al. Nat Commun, 2017 Jan 10. PMID 28071751, Free PMC Article
  2. ThiC is an [Fe-S] cluster protein that requires AdoMet to generate the 4-amino-5-hydroxymethyl-2-methylpyrimidine moiety in thiamin synthesis. Martinez-Gomez NC, et al. Biochemistry, 2008 Sep 2. PMID 18686975, Free PMC Article
  3. Biosynthesis of the thiamin pyrimidine: the reconstitution of a remarkable rearrangement reaction. Lawhorn BG, et al. Org Biomol Chem, 2004 Sep 7. PMID 15326535
  4. Role of a hairpin-stabilized pause in the Escherichia coli thiC riboswitch function. Chauvier A, et al. RNA Biol, 2019 Aug. PMID 31081713, Free PMC Article
  5. Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression. Winkler W, et al. Nature, 2002 Oct 31. PMID 12410317

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables carbon-carbon lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in thiamine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within thiamine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
phosphomethylpyrimidine synthase
NP_418422.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4194204..4196099 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418422.1 phosphomethylpyrimidine synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418422.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827YKT8
    Conserved Domains (1) summary
    PRK09284
    Location:9 → 626
    PRK09284; thiamine biosynthesis protein ThiC; Provisional
Nucleotide Protein
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