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thiH 2-iminoacetate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948494, updated on 4-Apr-2024

Summary

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Gene symbol
thiH
Gene description
2-iminoacetate synthase
Primary source
ASAP:ABE-0013048
Locus tag
b3990
See related
ECOCYC:EG11590
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3981; thiB
Summary
Anaerobically, glycine probably cannot be oxidized to form the probable thiamine biosynthetic intermediate dehydroglycine, so it is derived from tyrosine by the action of the tyrosine lyase ThiH; the products of the in vitro ThiH reaction are para-cresol and hydroglycine (Kriek, 2007). [More information is available at EcoGene: EG11590]. The ThiGH complex catalyzes the conversion of 1-deoxy-D-xylulose 5-phosphate to a thiazole as part of thiamine synthesis. [More information is available at EcoCyc: EG11590].
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Genomic context

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Sequence:
NC_000913.3 (4190735..4191868, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YjaZ Neighboring gene ncRNA Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene thiamine phosphate synthase Neighboring gene phosphomethylpyrimidine synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mutational analysis of ThiH, a member of the radical S-adenosylmethionine (AdoMet) protein superfamily. Martinez-Gomez NC, et al. J Biol Chem, 2004 Sep 24. PMID 15271986
  2. [Tyrosine as a factor of biosynthesis of the thiazole moiety of thiamin in Escherichia coli]. Estramareix B, et al. Biochim Biophys Acta, 1972 Jul 19. PMID 4562671
  3. Catalytic activity of the anaerobic tyrosine lyase required for thiamine biosynthesis in Escherichia coli. Challand MR, et al. J Biol Chem, 2010 Feb 19. PMID 19923213, Free PMC Article
  4. Thiamine biosynthesis in Escherichia coli: identification of the intermediate and by-product derived from tyrosine. Kriek M, et al. Angew Chem Int Ed Engl, 2007. PMID 17969213
  5. Isolation of 5-(beta-hydroxyethl)-4-methylthiazole-2-carboxylic acid, a metabolite related to thiamine biosynthesis in Escherichia coli. Estramareix B, et al. Biochem Biophys Res Commun, 1980 Aug 14. PMID 6998471

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results describe the substrates and purified proteins required for Escherichia coli thiazole synthase subunits ThiG and ThiH in vitro.
    Title: Thiazole synthase from Escherichia coli: an investigation of the substrates and purified proteins required for activity in vitro.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2-iminoacetate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2-iminoacetate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular nitrogen compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in heterocycle biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in organic cyclic compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in organonitrogen compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in sulfur compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within thiamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in thiamine diphosphate biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in water-soluble vitamin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of 2-iminoacetate synthase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
2-iminoacetate synthase
NP_418417.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4190735..4191868 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418417.1 2-iminoacetate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418417.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AF67
    Conserved Domains (1) summary
    PRK09240
    Location:2 → 373
    thiH; 2-iminoacetate synthase ThiH
Nucleotide Protein
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