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metH cobalamin-dependent methionine synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948522, updated on 2-May-2024

Summary

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Gene symbol
metH
Gene description
cobalamin-dependent methionine synthase
Primary source
ASAP:ABE-0013140
Locus tag
b4019
See related
ECOCYC:EG10587
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4011
Summary
[More information is available at EcoCyc: EG10587].
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Genomic context

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Sequence:
NC_000913.3 (4223828..4227511)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene isocitrate dehydrogenase kinase/phosphatase Neighboring gene regulator of acetyl CoA synthetase Neighboring gene DNA-binding transcriptional repressor IclR Neighboring gene putative inorganic phosphate export protein YjbB Neighboring gene peptidase E

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Role of heat-shock proteins and cobalamine in maintaining methionine synthase activity. Grabowski M, et al. Acta Biochim Pol, 2012. PMID 23251906
  2. Insights into the reactivation of cobalamin-dependent methionine synthase. Koutmos M, et al. Proc Natl Acad Sci U S A, 2009 Nov 3. PMID 19846791, Free PMC Article
  3. Spectroscopic study of the cobalamin-dependent methionine synthase in the activation conformation: effects of the Y1139 residue and S-adenosylmethionine on the B12 cofactor. Liptak MD, et al. J Am Chem Soc, 2008 Dec 3. PMID 19006389, Free PMC Article
  4. Ligand trans influence governs conformation in cobalamin-dependent methionine synthase. Fleischhacker AS, et al. Biochemistry, 2007 Oct 30. PMID 17924667, Free PMC Article
  5. Probing the role of the histidine 759 ligand in cobalamin-dependent methionine synthase. Liptak MD, et al. Biochemistry, 2007 Jul 10. PMID 17567043, Free PMC Article

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. describe an x-ray structure of the mutant fragment in the reactivation conformation
    Title: A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables cobalamin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cobalamin binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methionine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methionine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methionine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in homocysteine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in homocysteine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in methionine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in methionine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in methionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methionine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in pteridine-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tetrahydrofolate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
cobalamin-dependent methionine synthase
NP_418443.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4223828..4227511
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418443.1 cobalamin-dependent methionine synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418443.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6PZC3
    Conserved Domains (1) summary
    PRK09490
    Location:1 → 1223
    metH; B12-dependent methionine synthase; Provisional
Nucleotide Protein
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