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malF maltose ABC transporter membrane subunit MalF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948532, updated on 11-Apr-2024

Summary

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Gene symbol
malF
Gene description
maltose ABC transporter membrane subunit MalF
Primary source
ASAP:ABE-0013195
Locus tag
b4033
See related
ECOCYC:EG10555
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4025
Summary
The maltose transport complex MalFGK(2) also transports maltodextrin. [More information is available at EcoGene: EG10555]. MalF is an integral membrane component of the maltose ABC transporter. [More information is available at EcoCyc: EG10555].
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Genomic context

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Sequence:
NC_000913.3 (4243531..4245075, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene D-xylose:H(+) symporter Neighboring gene ncRNA Neighboring gene maltose ABC transporter membrane subunit MalG Neighboring gene maltose ABC transporter periplasmic binding protein Neighboring gene maltose ABC transporter ATP binding subunit

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. NMR assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter. Jacso T, et al. Biomol NMR Assign, 2009 Jun. PMID 19636938
  2. Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system. Shuman HA, et al. J Biol Chem, 1980 Jan 10. PMID 6765939
  3. Conversion of beta-galactosidase to a membrane-bound state by gene fusion. Silhavy TJ, et al. Proc Natl Acad Sci U S A, 1976 Oct. PMID 790385, Free PMC Article
  4. Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF. Licht A, et al. Res Microbiol, 2019 Jan-Feb. PMID 30193862
  5. Studies of the maltose transport system reveal a mechanism for coupling ATP hydrolysis to substrate translocation without direct recognition of substrate. Gould AD, et al. J Biol Chem, 2010 Apr 9. PMID 20147285, Free PMC Article

See all (170) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Bdellovibrio bacteriovorus and the E. coli MelF share substrate specificity for maltose and linear dextrins.
    Title: Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF.
  2. Data indicate that MalFGK2 transporter is comprised of two membrane proteins, MalF and MalG, and the nucleotide-binding domain, which controls the conformation of MalFG, is composed of the homodimeric MalK subunit.
    Title: Formation of a Chloride-conducting State in the Maltose ATP-binding Cassette (ABC) Transporter.
  3. Data suggest that the binding and conformational change of maltose transporter (MalFGK2) may provide some insights into the understanding of the mechanism of ABC transporters.
    Title: Allosteric transitions of the maltose transporter studied by an elastic network model.
  4. The molecular entity that interacts with MalT is not free MalK, but the maltose transporter, MalFGK(2) , which sequesters MalT to the membrane.
    Title: The ABC transporter MalFGK(2) sequesters the MalT transcription factor at the membrane in the absence of cognate substrate.
  5. using x-ray crystallography, study captured the maltose transporter in an intermediate step between the inward- and outward-facing states
    Title: Crystal structure of the maltose transporter in a pretranslocation intermediate state.
  6. analysis of the MalFGK2 mechanism for coupling ATP hydrolysis to substrate translocation without direct recognition of substrate
    Title: Studies of the maltose transport system reveal a mechanism for coupling ATP hydrolysis to substrate translocation without direct recognition of substrate.
  7. YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex
    Title: Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC.
  8. substrate availability is communicated from MalE to the MalK dimer via extracytoplasmic loops of MalFG
    Title: Maltose binding protein (MalE) interacts with periplasmic loops P2 and P1 respectively of the MalFG subunits of the maltose ATP binding cassette transporter (MalFGK(2)) from Escherichia coli/Salmonella during the transport cycle.
  9. 2.8-A crystal structure of the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP
    Title: Crystal structure of a catalytic intermediate of the maltose transporter.
  10. ATP-driven MalK dimer closure and reopening are crucial steps in the translocation cycle of the intact maltose transporter MalFGK2
    Title: ATP-driven MalK dimer closure and reopening and conformational changes of the "EAA" motifs are crucial for function of the maltose ATP-binding cassette transporter (MalFGK2).
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ABC-type maltose transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ABC-type maltose transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in maltodextrin transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within maltodextrin transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in maltodextrin transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in maltose transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within maltose transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of maltose transport NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of transmembrane transport NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in organic substance transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of ATP-binding cassette (ABC) transporter complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of enzyme IIA-maltose transporter complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of maltose transport complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of maltose transport complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of maltose transport complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
maltose ABC transporter membrane subunit MalF
NP_418457.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4243531..4245075 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418457.1 maltose ABC transporter membrane subunit MalF [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418457.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A377A981, A0AA90J8Y4
    Conserved Domains (1) summary
    PRK10999
    Location:1 → 514
    malF; maltose ABC transporter permease MalF
Nucleotide Protein
Heading Accession and Version

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