malE maltose ABC transporter periplasmic binding protein [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: malE
Gene description: maltose ABC transporter periplasmic binding protein
Primary source: ASAP:ABE-0013200
Locus tag: b4034
See related: ECOCYC:EG10554
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK4026
Summary: Biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG10554]. In addition to being the periplasmic substrate-binding component of the maltose ABC transporter, MalE with bound substrate is also able to bind to the chemoreceptor Tar to induce chemotaxis toward maltose. [More information is available at EcoCyc: EG10554].
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Genomic context

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Sequence:: NC_000913.3 (4245229..4246419, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A useful epitope tag derived from maltose binding protein.
Title: A useful epitope tag derived from maltose binding protein.
Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein.
Title: Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein.
MelF activities of the B. bacteriovorus transporter were comparable to those of the E. coli system assayed at a 1:1 M ratio of MalE to the transport complex.
Title: Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF.
Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay.
Title: Folding of maltose binding protein outside of and in GroEL.
Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW]
Title: The remarkable solubility-enhancing power of Escherichia coli maltose-binding protein.
Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP
Title: Full engagement of liganded maltose-binding protein stabilizes a semi-open ATP-binding cassette dimer in the maltose transporter.
activates mouse macrophages and polarizes them into M1 macrophages
Title: Escherichia coli maltose-binding protein activates mouse peritoneal macrophages and induces M1 polarization via TLR2/4 in vivo and in vitro.
MalE mutant causes elevated levels of phosphorylated CpxR and it does not affect the phosphorylation level of CpxA.
Title: Misfolded maltose binding protein MalE219 induces the CpxRA envelope stress response by stimulating phosphoryl transfer from CpxA to CpxR.
tat mutants are able to transport maltose in the absence of malE.
Title: Escherichia coli tat mutant strains are able to transport maltose in the absence of an active malE gene.
These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB.
Title: Kinetics and energetics of the translocation of maltose binding protein folding mutants.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables carbohydrate transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables maltose binding	IBA Inferred from Biological aspect of Ancestor more info
enables maltose binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
involved_in carbohydrate transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in carbohydrate transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within carbohydrate transport	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within detection of maltose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maltodextrin transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in maltodextrin transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within maltodextrin transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maltose transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within maltose transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within maltose transport	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within maltose transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in organic substance transport	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of ATP-binding cassette (ABC) transporter complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing	IBA Inferred from Biological aspect of Ancestor more info
part_of ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing	IGI Inferred from Genetic Interaction more info	PubMed
part_of maltose transport complex	IPI Inferred from Physical Interaction more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in outer membrane-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in outer membrane-bounded periplasmic space	IEA Inferred from Electronic Annotation more info
located_in periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic space	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: maltose ABC transporter periplasmic binding protein

NP_418458.1

EC 7.5.2.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4245229..4246419 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418458.1 maltose ABC transporter periplasmic binding protein [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418458.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A6C9Q5Z7

Conserved Domains (1) summary

PRK09474
Location:1 → 396

malE; maltose/maltodextrin ABC transporter substrate-binding protein MalE