dgkA diacylglycerol kinase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: dgkA
Gene description: diacylglycerol kinase
Primary source: ASAP:ABE-0013235
Locus tag: b4042
See related: ECOCYC:EG10224
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK4034
Summary: DgkA is an integral membrane protein. [More information is available at EcoGene: EG10224]. Diacylglycerol kinase is the smallest known kinase. [More information is available at EcoCyc: EG10224].
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Genomic context

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Sequence:: NC_000913.3 (4256637..4257005)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The active site of dgkA including glutamates and an aspartate plays roles in substrate binding and catalysis.
Title: Ternary structure reveals mechanism of a membrane diacylglycerol kinase.
PlsB activation by RpoE is concomitant with a reduced DgkA amount.
Title: Antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in Escherichia coli.
Magic angle spinning NMR allows simultaneous & direct detection of both ATP hydrolysis and diacylglycerol phosphorylation for DGK; data yield conclusions about intersubunit cooperativity, reaction stoichiometries and phosphoryl transfer mechanism.
Title: Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
determination of the three-dimensional structure of the diacylglycerol kinase homotrimer with the use of solution nuclear magnetic resonance
Title: Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase.
Diacylglycerol kinase from Escherichia coli contains three transmembrane alpha-helices per monomer. We used fluorescence techniques to define region of first transmembrane helix that spans the hydrophobic core of the reconstituted membranes.
Title: A fluorescence method to define transmembrane alpha-helices in membrane proteins: studies with bacterial diacylglycerol kinase.
N-terminus verified by Edman degradation on mature peptide
Title: sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis.
Results describe the mechanism of folding and insertion into lipid bilayers of Escherichia coli diacylglycerol kinase from a partially denatured state in urea.
Title: Insertion kinetics of a denatured alpha helical membrane protein into phospholipid bilayer vesicles.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in lipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phosphatidic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to UV	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: diacylglycerol kinase

NP_418466.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4256637..4257005

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418466.1 diacylglycerol kinase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418466.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9ABV9

Conserved Domains (1) summary

COG0818
Location:1 → 121

DgkA; Diacylglycerol kinase [Lipid transport and metabolism]