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nrfB periplasmic nitrite reductase penta-heme c-type cytochrome [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948573, updated on 11-Apr-2024

Summary

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Gene symbol
nrfB
Gene description
periplasmic nitrite reductase penta-heme c-type cytochrome
Primary source
ASAP:ABE-0013344
Locus tag
b4071
See related
ECOCYC:EG11945
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4064; yjcI
Summary
SignalP strongly predicts a signal of 32 aa (34 aa if a GTG start at -2 is used), as previously noted (Clarke, 2004), but N-terminal sequencing found a cleavage after 25 residues (Clarke, 2007). [More information is available at EcoGene: EG11945]. The nrfB gene encodes a cytochrome C that is believed to be the direct electron donor for cytochrome C552 in the formate-dependent reduction of nitrite to ammonia. [More information is available at EcoCyc: EG11945].
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Genomic context

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Sequence:
NC_000913.3 (4289245..4289811)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YtcB Neighboring gene cytochrome c552 nitrite reductase Neighboring gene putative menaquinol-cytochrome c reductase 4Fe-4S subunit Neighboring gene putative menaquinol-cytochrome c reductase subunit NrfD Neighboring gene putative cytochrome c-type biogenesis protein NrfE Neighboring gene putative formate-dependent nitrite reductase complex subunit NrfF

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA. Clarke TA, et al. Biochem J, 2007 Aug 15. PMID 17521287, Free PMC Article
  2. Purification and spectropotentiometric characterization of Escherichia coli NrfB, a decaheme homodimer that transfers electrons to the decaheme periplasmic nitrite reductase complex. Clarke TA, et al. J Biol Chem, 2004 Oct 1. PMID 15280383
  3. Role of a conserved glutamine residue in tuning the catalytic activity of Escherichia coli cytochrome c nitrite reductase. Clarke TA, et al. Biochemistry, 2008 Mar 25. PMID 18311941
  4. The nitric oxide reductase activity of cytochrome c nitrite reductase from Escherichia coli. van Wonderen JH, et al. J Biol Chem, 2008 Apr 11. PMID 18245085
  5. Respiratory detoxification of nitric oxide by the cytochrome c nitrite reductase of Escherichia coli. Poock SR, et al. J Biol Chem, 2002 Jun 28. PMID 11960983

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
    Title: The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables electron transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nitrite reductase (cytochrome, ammonia-forming) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables oxidoreductase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in anaerobic respiration IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
periplasmic nitrite reductase penta-heme c-type cytochrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4289245..4289811
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418495.2 periplasmic nitrite reductase penta-heme c-type cytochrome [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418495.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A0H3JMG5, A0A1X3J930, C3SH92, F4VM63
    Conserved Domains (1) summary
    PRK11659
    Location:4 → 186
    PRK11659; cytochrome c nitrite reductase pentaheme subunit; Provisional
Nucleotide Protein
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Gene LinkOut

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