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melB melibiose:H(+)/Na(+)/Li(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948635, updated on 11-Apr-2024

Summary

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Gene symbol
melB
Gene description
melibiose:H(+)/Na(+)/Li(+) symporter
Primary source
ASAP:ABE-0013495
Locus tag
b4120
See related
ECOCYC:EG10578
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4113; mel-4
Summary
Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG10578]. MelB is a melibiose-cation cotransport protein belonging to the Glycoside-Pentoside-Hexuronide:Cation Symporter Family (GPH) . [More information is available at EcoCyc: EG10578].
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Genomic context

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Sequence:
NC_000913.3 (4343369..4344790)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator MelR Neighboring gene alpha-galactosidase Neighboring gene DUF2238 domain-containing inner membrane protein YjdF Neighboring gene fumarase B

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES. Fuerst O, et al. J Biol Chem, 2015 Jun 26. PMID 25971963, Free PMC Article
  2. A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters. Yousef MS, et al. Proc Natl Acad Sci U S A, 2009 Sep 8. PMID 19706416, Free PMC Article
  3. Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5. León X, et al. Biophys J, 2009 Jun 17. PMID 19527646, Free PMC Article
  4. Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane. Garcia-Celma JJ, et al. Langmuir, 2008 Aug 5. PMID 18572928
  5. FTIR spectroscopy of secondary-structure reorientation of melibiose permease modulated by substrate binding. Dave N, et al. Biophys J, 2008 May 1. PMID 18024501, Free PMC Article

See all (61) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lys-377 and Ile-22 of melB are crucial for the substrates binding.
    Title: The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES.
  2. Data show that the regulatory protein IIA(Glc) directly binds to melibiose permease (MelB) with dissociation constant value 25 muM.
    Title: Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity.
  3. MelB-N-ethylmaleimide difference spectra are proportionally less disrupted than the R141C ones
    Title: Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5.
  4. Data describe the three-dimensional structure of melibiose permease at 10 angstrom resolution in the membrane plane with cryo-electron microscopy from two-dimensional crystals.
    Title: Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy.
  5. Data suggest that loop 4-5 of melibiose permease contributes to coordinated interactions between ion and sugar binding sites, and participates in an electrogenic conformational transition essential for coupled substrate translocation.
    Title: The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding.
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Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glycoside-pentoside-hexuronide:cation symporter activity RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
enables melibiose:monoatomic cation symporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables melibiose:sodium symporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylgalactoside transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables symporter activity IEA
Inferred from Electronic Annotation
more info
  
enables transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in carbohydrate transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in carbohydrate transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within melibiose transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within methylgalactoside transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in organic substance transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in organic substance transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
melibiose:H(+)/Na(+)/Li(+) symporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4343369..4344790
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418544.2 melibiose:H(+)/Na(+)/Li(+) symporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418544.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2W6P8E3, A0A5B9ABA5, A0A8S7I3I8
    Conserved Domains (1) summary
    PRK10429
    Location:1 → 473
    PRK10429; melibiose:sodium transporter MelB
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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Other Literature Sources
Molecular Biology Databases