U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

dcuB anaerobic C4-dicarboxylate transporter DcuB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948641, updated on 6-Oct-2023

Summary

Go to the top of the page Help
Gene symbol
dcuB
Gene description
anaerobic C4-dicarboxylate transporter DcuB
Primary source
ASAP:ABE-0013503
Locus tag
b4123
See related
ECOCYC:EG10006
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4116; genF
Summary
IT (Ion Transporter) superfamily (Prakash, 2003). [More information is available at EcoGene: EG10006]. The DcuB transporter is one of three transporters known to be responsible for the uptake of C4-dicarboxylates such as fumarate under anaerobic conditions. [More information is available at EcoCyc: EG10006].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (4347404..4348744, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2238 domain-containing inner membrane protein YjdF Neighboring gene fumarase B Neighboring gene DNA-binding transcriptional activator DcuR Neighboring gene sensor histidine kinase DcuS Neighboring gene PF06902 family protein YjdI

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut. Lourenço M, et al. PLoS Genet, 2016 Nov. PMID 27812114, Free PMC Article
  2. The fumarate/succinate antiporter DcuB of Escherichia coli is a bifunctional protein with sites for regulation of DcuS-dependent gene expression. Kleefeld A, et al. J Biol Chem, 2009 Jan 2. PMID 18957436
  3. Influence of C(4) -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli. Karapetyan L, et al. IUBMB Life, 2020 Aug. PMID 32277802
  4. Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR. Wörner S, et al. J Bacteriol, 2018 Feb 15. PMID 29203472, Free PMC Article
  5. Transport of C4-dicarboxylates by anaerobically grown Escherichia coli. Energetics and mechanism of exchange, uptake and efflux. Engel P, et al. Eur J Biochem, 1994 Jun 1. PMID 8020497

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Influence of C4 -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli.
    Title: Influence of C(4) -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli.
  2. The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models.
    Title: Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR.
  3. in vivo competitive assays showed that one of the second steps (focA) exhibited positive epistasis with the first, while another (dcuB) exhibited negative epistasis. The data shows that strong effect adaptive mutations continuously recur in gut commensal bacterial species.
    Title: A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut.
  4. Deleting both dcuB and dcuC genes resulted in 90% decrease of succinate titer, suggesting that DcuB and DcuC were the main transporters for succinate efflux.
    Title: Activating C4-dicarboxylate transporters DcuB and DcuC for improving succinate production.
Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables C4-dicarboxylate transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables C4-dicarboxylate transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables succinate:fumarate antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables symporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within C4-dicarboxylate transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in C4-dicarboxylate transport IEA
Inferred from Electronic Annotation
more info
  
involved_in fumarate transport IEA
Inferred from Electronic Annotation
more info
  
involved_in succinate transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
anaerobic C4-dicarboxylate transporter DcuB

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4347404..4348744 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418547.1 anaerobic C4-dicarboxylate transporter DcuB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418547.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AD81, A0A7D7KDE4, A0A8S7JZC2
    Conserved Domains (1) summary
    PRK12489
    Location:38 → 444
    PRK12489; anaerobic C4-dicarboxylate transporter; Reviewed
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.