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rsgA ribosome small subunit-dependent GTPase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948674, updated on 4-Apr-2024

Summary

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Gene symbol
rsgA
Gene description
ribosome small subunit-dependent GTPase A
Primary source
ASAP:ABE-0013626
Locus tag
b4161
See related
ECOCYC:G7841
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4157; yjeQ
Summary
rsgA(yjeQ) has been reported to be an essential gene, but deletion mutants have also been reported to grow slowly. [More information is available at EcoGene: EG12479]. Based on genetic interactions, RgsA/YjeQ is thought to play a role in late ribosome biogenesis . [More information is available at EcoCyc: G7841].
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Genomic context

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Sequence:
NC_000913.3 (4390457..4391509, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene miniconductance mechanosensitive channel MscM Neighboring gene phosphatidylserine decarboxylase proenzyme Neighboring gene oligoribonuclease Neighboring gene tRNA-Gly

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly. Razi A, et al. Proc Natl Acad Sci U S A, 2017 Apr 25. PMID 28396444, Free PMC Article
  2. Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor. Jomaa A, et al. RNA, 2011 Nov. PMID 21960487, Free PMC Article
  3. Structural basis for the function of a small GTPase RsgA on the 30S ribosomal subunit maturation revealed by cryoelectron microscopy. Guo Q, et al. Proc Natl Acad Sci U S A, 2011 Aug 9. PMID 21788480, Free PMC Article
  4. Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy. Jomaa A, et al. RNA, 2011 Apr. PMID 21303937, Free PMC Article
  5. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. Hase Y, et al. RNA, 2009 Sep. PMID 19620234, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. This finding indicates that, along with acting as an assembly factor, YjeQ has a role as a checkpoint protein, consisting of testing the proofreading ability of the 30S subunit. The structure also informs the mechanism by which YjeQ implements the release from the 30S subunit of a second assembly factor, called RbfA.
    Title: The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly.
  2. Results shows that YjeQ promotes the release of RbfA through the C-terminal helix in its zinc-finger domain.
    Title: The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.
  3. costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit
    Title: Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.
  4. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells.
    Title: Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells.
  5. The work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis.
    Title: Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis.
  6. RsgA may be close contact with the A site of the decoding center of the small subunit of the ribosome.
    Title: Interaction between RsgA and the ribosome.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GDP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables rRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within ribosomal small subunit assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosomal small subunit biogenesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within ribosomal small subunit biogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ribosome small subunit-dependent GTPase A
NP_418585.4

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4390457..4391509 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418585.4 ribosome small subunit-dependent GTPase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418585.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7H9SCA2
    Conserved Domains (1) summary
    PRK12288
    Location:1 → 350
    PRK12288; small ribosomal subunit biogenesis GTPase RsgA
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials