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amiB N-acetylmuramoyl-L-alanine amidase B [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948683, updated on 23-Nov-2023

Summary

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Gene symbol
amiB
Gene description
N-acetylmuramoyl-L-alanine amidase B
Primary source
ASAP:ABE-0013653
Locus tag
b4169
See related
ECOCYC:EG11363
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4165; yjeD
Summary
First 22 aa are predicted to be a type I signal peptide. [More information is available at EcoGene: EG11363]. Escherichia coli contains three N-acetylmuramyl-L-alanine amidases named AmiA, B, and C involved in cell division by splitting the murein septum. [More information is available at EcoCyc: EG11363].
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Genomic context

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Sequence:
NC_000913.3 (4396065..4397402)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein YjeV Neighboring gene epoxyqueuosine reductase Neighboring gene NAD(P)HX epimerase/NAD(P)HX dehydratase Neighboring gene N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit Neighboring gene DNA mismatch repair protein MutL Neighboring gene tRNA dimethylallyltransferase Neighboring gene RNA-binding protein Hfq

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division. Yang DC, et al. Mol Microbiol, 2012 Aug. PMID 22715947, Free PMC Article
  2. A fail-safe mechanism in the septal ring assembly pathway generated by the sequential recruitment of cell separation amidases and their activators. Peters NT, et al. J Bacteriol, 2011 Sep. PMID 21764913, Free PMC Article
  3. Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium. Tsui HT, et al. Nucleic Acids Res, 1992 May 11. PMID 1594459, Free PMC Article
  4. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. Beck BD, et al. J Bacteriol, 1976 Jun. PMID 780345, Free PMC Article
  5. The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase. Tsui HC, et al. Mol Microbiol, 1994 Jan. PMID 7511774

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Poorly regulated amiB mutants were isolated encoding lytic AmiB variants with elevated basal PG hydrolase activities in vitro.
    Title: A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division.
  2. The authors report that AmiB and EnvC are recruited to division sites independently of one another. The same is also true for AmiC and NlpD.
    Title: A fail-safe mechanism in the septal ring assembly pathway generated by the sequential recruitment of cell separation amidases and their activators.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables N-acetylmuramoyl-L-alanine amidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-acetylmuramoyl-L-alanine amidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables N-acetylmuramoyl-L-alanine amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within FtsZ-dependent cytokinesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in cytokinesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptidoglycan catabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
N-acetylmuramoyl-L-alanine amidase B
NP_418590.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4396065..4397402
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418590.1 N-acetylmuramoyl-L-alanine amidase B [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418590.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5ZBW8
    Conserved Domains (1) summary
    PRK10431
    Location:1 → 445
    PRK10431; N-acetylmuramoyl-l-alanine amidase II; Provisional
Nucleotide Protein
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