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pepA aminopeptidase A/I [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948791, updated on 4-Apr-2024

Summary

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Gene symbol
pepA
Gene description
aminopeptidase A/I
Primary source
ASAP:ABE-0013953
Locus tag
b4260
See related
ECOCYC:EG10694
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
carP; ECK4253; xerB
Summary
PepA has a broad specificity for successive removal of many N-terminal amino acids, although leucine is preferred as the residue N-terminal to the cleavage site. [More information is available at EcoGene: EG10694]. Aminopeptidase A/I is a peptidase that binds DNA and is required for maintenance of plasmid monomers, which is critical for proper plasmid segregation . [More information is available at EcoCyc: EG10694].
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Genomic context

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Sequence:
NC_000913.3 (4484440..4485951, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene valine--tRNA ligase Neighboring gene DNA polymerase III subunit chi Neighboring gene protein YtgB Neighboring gene protein YtgA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The trigger enzyme PepA (aminopeptidase A) of Escherichia coli, a transcriptional repressor that generates positive supercoiling. Nguyen Le Minh P, et al. FEBS Lett, 2016 Jun. PMID 27213286
  2. Insights into the architecture and stoichiometry of Escherichia coli PepA*DNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy. Minh PN, et al. Nucleic Acids Res, 2009 Apr. PMID 19136463, Free PMC Article
  3. Control of Cre recombination by regulatory elements from Xer recombination systems. Gourlay SC, et al. Mol Microbiol, 2004 Apr. PMID 15049810
  4. Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. Charlier D, et al. J Mol Biol, 2000 Sep 15. PMID 10970742
  5. A bicarbonate ion as a general base in the mechanism of peptide hydrolysis by dizinc leucine aminopeptidase. Sträter N, et al. Proc Natl Acad Sci U S A, 1999 Sep 28. PMID 10500145, Free PMC Article

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. in vitro topology studies performed with DNA minicircles demonstrate that PepA binding constrains a single positive supercoil in the carP1 control region. Such a topological event is understood to constitute an impediment to transcription initiation and may serve as a mechanism to regulate gene expression.
    Title: The trigger enzyme PepA (aminopeptidase A) of Escherichia coli, a transcriptional repressor that generates positive supercoiling.
  2. Data show that PepA can effectively process heptapeptide-nucleotide microcin C (McC).
    Title: Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
  3. N-terminus verified by Edman degradation on complete protein
    Title: xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.
  4. Random PepA mutants unable to support efficient Xer recombination are reported.
    Title: Mutagenesis of PepA suggests a new model for the Xer/cer synaptic complex.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables aminopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables aminopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables aminopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables manganese ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloaminopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables metalloexopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables transcription antitermination factor activity, DNA binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within peptide catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within plasmid maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within plasmid recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in transcription antitermination IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
aminopeptidase A/I
NP_418681.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4484440..4485951 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418681.1 aminopeptidase A/I [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418681.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A828GML7, A0A8S7J8J5
    Conserved Domains (1) summary
    PRK00913
    Location:1 → 495
    PRK00913; multifunctional aminopeptidase A; Provisional
Nucleotide Protein
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Other Literature Sources
Molecular Biology Databases
Research Materials