› 1t2p

X-ray diffraction
2Å resolution

Crystal structure of Sortase A from Staphylococcus aureus

Released:
DOI: 10.2210/pdb1t2p/pdb
PDB entry 1t2p coloured by chain, front view.
PDB entry 1t2p coloured by chain, side view.
PDB entry 1t2p coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of Staphylococcus aureus sortase A and its substrate complex.
Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SV
J Biol Chem 279 31383-9 (2004)
PMID: 15117963

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LPXTG specific sortase A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 146 amino acids
Theoretical weight: 16.49 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S446 (Residues: 61-206; Coverage: 71%)
Gene names: EP54_00580, EQ90_03180, FAF17_05955, GO814_08865, GO942_11165, GQX37_12930, GZ163_10635, HMPREF3211_00152, NCTC13131_00030, SAMEA2078260_01136, SAMEA2078588_01162, SAMEA2080344_00650, SAMEA2081063_00930, SAMEA4008575_00757, SAMEA70146418_00206, srtA
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 46.553Å b: 81.907Å c: 54.912Å
α: 90° β: 103.75° γ: 90°
R-values:
R R work R free
0.221 0.209 0.257
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Sortase enzymes in Gram-positive bacteria.
Spirig et al. (2011)
20 more

45 mentions without citation

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.
Marraffini et al. (2006)
44 more