› 3ziz

X-ray diffraction
1.4Å resolution

Crystal structure of Podospora anserina GH5 beta-(1,4)-mannanase

Released:
DOI: 10.2210/pdb3ziz/pdb
PDB entry 3ziz coloured by chain, front view.
PDB entry 3ziz coloured by chain, side view.
PDB entry 3ziz coloured by chain, top view.
Source organism: Podospora anserina
Primary publication:
Structural and biochemical analyses of glycoside hydrolase families 5 and 26 β-(1,4)-mannanases from Podospora anserina reveal differences upon manno-oligosaccharide catalysis.
Couturier M, Roussel A, Rosengren A, Leone P, Stålbrand H, Berrin JG
J Biol Chem 288 14624-14635 (2013)
PMID: 23558681

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase A Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.5 KDa
Source organism: Podospora anserina
Expression system: Komagataella pastoris
UniProt:
  • Canonical: B2B3C0 (Residues: 18-373; Coverage: 100%)
Gene names: PODANS_6_490, Pa_6_490
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 56.87Å b: 57.9Å c: 97.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.172
Expression system: Komagataella pastoris

Quick links

Citations

9 review citations

Mannan biotechnology: from biofuels to health.
Yamabhai et al. (2016)
8 more

7 mentions without citation

Structural and biochemical analyses of glycoside hydrolase families 5 and 26 β-(1,4)-mannanases from Podospora anserina reveal differences upon manno-oligosaccharide catalysis.
Couturier et al. (2013)
6 more