› 4zxe

X-ray diffraction
1.4Å resolution

X-ray crystal structure of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5.

Released:
DOI: 10.2210/pdb4zxe/pdb
PDB entry 4zxe coloured by chain, front view.
PDB entry 4zxe coloured by chain, side view.
PDB entry 4zxe coloured by chain, top view.
Source organism: Paenibacillus fukuinensis
Primary publication:
Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase.
Shinya S, Nishimura S, Kitaoku Y, Numata T, Kimoto H, Kusaoke H, Ohnuma T, Fukamizo T
Biochem J 473 1085-95 (2016)
PMID: 26936968

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 137 amino acids
Theoretical weight: 15.05 KDa
Source organism: Paenibacillus fukuinensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93IE7 (Residues: 530-659; Coverage: 16%)
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand EDO 18 x EDO
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 42.054Å b: 45.591Å c: 57.581Å
α: 111.69° β: 105.36° γ: 97.92°
R-values:
R R work R free
0.145 0.143 0.172
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Interaction between chitosan and its related enzymes: A review.
Shinya et al. (2017)
2 more

1 mention without citation

Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.
Xu et al. (2022)