› 5mwn

X-ray diffraction
2.2Å resolution

Structure of the EAEC T6SS component TssK N-terminal domain in complex with llama nanobodies nbK18 and nbK27

Released:
DOI: 10.2210/pdb5mwn/pdb
PDB entry 5mwn coloured by chain, front view.
PDB entry 5mwn coloured by chain, side view.
PDB entry 5mwn coloured by chain, top view.
Source organisms:
Primary publication:
Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex.
Nguyen VS, Logger L, Spinelli S, Legrand P, Huyen Pham TT, Nhung Trinh TT, Cherrak Y, Zoued A, Desmyter A, Durand E, Roussel A, Kellenberger C, Cascales E, Cambillau C
Nat Microbiol 2 17103 (2017)
PMID: 28650463

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-211719 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Type VI secretion protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 315 amino acids
Theoretical weight: 35.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
llama nanobody raised against TssK, nbK18 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 125 amino acids
Theoretical weight: 13.71 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins
llama nanobody raised against TssK, nbK27 Chain: N
Molecule details ›
Chain: N
Length: 125 amino acids
Theoretical weight: 13.63 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 143.31Å b: 150.28Å c: 90.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.22
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

7 review citations

Contractile injection systems of bacteriophages and related systems.
Taylor et al. (2018)
6 more

2 mentions without citation

Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.
Xu et al. (2022)
1 more