› 6pal

X-ray diffraction
1.82Å resolution

Bacteroides uniformis endo-laminarinase BuGH158 from the beta(1,3)-glucan utilization locus

Released:
DOI: 10.2210/pdb6pal/pdb
PDB entry 6pal coloured by chain, front view.
PDB entry 6pal coloured by chain, side view.
PDB entry 6pal coloured by chain, top view.
Source organism: Bacteroides uniformis
Primary publication:
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides.
Déjean G, Tamura K, Cabrera A, Jain N, Pudlo NA, Pereira G, Viborg AH, Van Petegem F, Martens EC, Brumer H
mBio 11 (2020)
PMID: 32265336

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 45.76 KDa
Source organism: Bacteroides uniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A412SRH5 (Residues: 25-429; Coverage: 100%)
Gene names: Bun01g_20700, DWW83_06800, GAQ34_06590, GAQ56_12305, GAQ59_09580

Ligands and Environments

2 bound ligands:
Ligand ACT 4 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 65.675Å b: 106.03Å c: 126.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.206
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Leveraging diet to engineer the gut microbiome.
Wolter et al. (2021)
5 more

3 mentions without citation

Molecular and phenotypic characterisation of novel Phaeoacremonium species isolated from esca diseased grapevines.
Essakhi et al. (2008)
2 more