TAFII250, Egr-1, and D-type cyclin expression in mice and neonatal rat cardiomyocytes treated with doxorubicin

Am J Physiol. 1999 Mar;276(3):H803-14. doi: 10.1152/ajpheart.1999.276.3.H803.

Abstract

Differential display identified that gene fragment HA220 homologous to the transcriptional activator factor II 250 (TAFII250) gene, or CCG1, was increased in hypertrophied rodent heart. To determine whether TAFII250 gene expression is modified after cardiac damage, we measured TAFII250 expression in vivo in mouse hearts after injection of the cardiotoxic agent doxorubicin (DXR) and in vitro in DXR-treated isolated rat neonatal cardiomyocytes. In vivo atrial natriuretic factor (ANF), beta-myosin heavy chain (beta-MHC), Egr-1, and TAFII250 expression increased with dose and time after a single DXR injection, but only ANF and beta-MHC expression were increased after multiple injections. After DXR treatment of neonatal cardiomyocytes we found decreased ANF, alpha-MHC, Egr-1, and TAFII250 expression. Expression of the TAFII250-regulated genes, the D-type cyclins, was increased after a single injection in adult mice and was decreased in DXR-treated cardiomyocytes. Thus expression of Erg-1, TAFII250, and the D-type cyclins is modulated after cardiotoxic damage in adult and neonatal heart.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Animals, Newborn / genetics
  • Cardiomegaly / physiopathology
  • Cyclin D
  • Cyclins / genetics*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Doxorubicin / pharmacology*
  • Early Growth Response Protein 1
  • Gene Expression / drug effects*
  • Gene Expression / physiology
  • Gene Expression Regulation / physiology
  • Heart / drug effects
  • Heart / physiopathology*
  • Histone Acetyltransferases
  • Immediate-Early Proteins*
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Myocardium / metabolism
  • Myocardium / pathology
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / physiology
  • RNA / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • TATA-Binding Protein Associated Factors*
  • Time Factors
  • Transcription Factor TFIID*
  • Transcription Factors / genetics*

Substances

  • Cyclin D
  • Cyclins
  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, mouse
  • Egr1 protein, rat
  • Immediate-Early Proteins
  • Nuclear Proteins
  • TATA-Binding Protein Associated Factors
  • Transcription Factor TFIID
  • Transcription Factors
  • RNA
  • Doxorubicin
  • Histone Acetyltransferases
  • TATA-binding protein associated factor 250 kDa

Associated data

  • GENBANK/AF081115
  • GENBANK/AF081116
  • GENBANK/AF081117
  • GENBANK/AH007553