Immunological evaluation of novel Mycobacterium tuberculosis culture filtrate proteins

FEMS Immunol Med Microbiol. 1999 Feb;23(2):159-64. doi: 10.1111/j.1574-695X.1999.tb01235.x.

Abstract

Culture filtrate from Mycobacterium tuberculosis contains molecules which can promote protective immunity to tuberculosis in animal models. Six novel proteins in the region of 17-29 kDa were purified and investigated for their immunological relevance in M. tuberculosis-infected mice, guinea pigs and tuberculosis patients. The proteins CFP17, CFP21, CFP25 and CFP29 were all identified as strong interferon-gamma inducers in M. tuberculosis-infected mice and in tuberculosis patients. The CFP21 protein is encoded in the genomic region RD-2 which is deleted from a number of BCG strains and the diagnostic potential of this antigen was evaluated.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Antigens, Bacterial / isolation & purification
  • BCG Vaccine / immunology
  • Cloning, Molecular
  • Conserved Sequence
  • Culture Media, Conditioned / chemistry*
  • Disease Models, Animal
  • Genome, Bacterial
  • Guinea Pigs
  • Humans
  • Hypersensitivity, Delayed
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / immunology
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Mycobacterium tuberculosis / chemistry
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / immunology*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Sequence Deletion
  • Tuberculosis / diagnosis
  • Tuberculosis / immunology*
  • Tuberculosis / microbiology
  • Vaccination

Substances

  • Antigens, Bacterial
  • BCG Vaccine
  • Culture Media, Conditioned
  • Recombinant Proteins
  • Interferon-gamma