Abstract
Culture filtrate from Mycobacterium tuberculosis contains molecules which can promote protective immunity to tuberculosis in animal models. Six novel proteins in the region of 17-29 kDa were purified and investigated for their immunological relevance in M. tuberculosis-infected mice, guinea pigs and tuberculosis patients. The proteins CFP17, CFP21, CFP25 and CFP29 were all identified as strong interferon-gamma inducers in M. tuberculosis-infected mice and in tuberculosis patients. The CFP21 protein is encoded in the genomic region RD-2 which is deleted from a number of BCG strains and the diagnostic potential of this antigen was evaluated.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology*
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Antigens, Bacterial / isolation & purification
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BCG Vaccine / immunology
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Cloning, Molecular
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Conserved Sequence
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Culture Media, Conditioned / chemistry*
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Disease Models, Animal
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Genome, Bacterial
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Guinea Pigs
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Humans
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Hypersensitivity, Delayed
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Interferon-gamma / biosynthesis
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Interferon-gamma / immunology
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Mice
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Molecular Sequence Data
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Molecular Weight
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Mycobacterium tuberculosis / chemistry
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / immunology*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification
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Sequence Deletion
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Tuberculosis / diagnosis
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Tuberculosis / immunology*
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Tuberculosis / microbiology
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Vaccination
Substances
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Antigens, Bacterial
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BCG Vaccine
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Culture Media, Conditioned
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Recombinant Proteins
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Interferon-gamma