Abstract
Human chondrosarcoma cells (HCS-TG) were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of human chondrosarcoma bearing an HSV-tk gene after treatment with ganciclovir. Chondrosarcoma cells bearing an HSV-tk gene were more sensitive than non-transduced cells. Coculturing with chondrosarcoma cells bearing both an HSV-tk gene (HCS-TG-tk) and lacZ gene (HCS-TG-Z) in various ratios showed a bystander effect. Chondrosarcoma implanted in nude mice were injected with HCS-TG-tk cells. After 4 weeks, the growth of tumors was significantly prevented.
MeSH terms
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Animals
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Antiviral Agents / pharmacology
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Avian Sarcoma Viruses / genetics*
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Bone Neoplasms / enzymology
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Bone Neoplasms / genetics
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Bone Neoplasms / pathology
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Bone Neoplasms / therapy*
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Cell Division / drug effects
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Chondrosarcoma / enzymology
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Chondrosarcoma / genetics
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Chondrosarcoma / pathology
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Chondrosarcoma / therapy*
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Coculture Techniques
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Dose-Response Relationship, Drug
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Ganciclovir / pharmacology
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Genetic Therapy / methods*
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Genetic Vectors
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Humans
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Male
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Mice
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Mice, Inbred ICR
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Mice, Nude
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Moloney murine leukemia virus / genetics*
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Neoplasm Transplantation
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Simplexvirus / enzymology*
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Thymidine Kinase / genetics*
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Transfection
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Tumor Cells, Cultured
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beta-Galactosidase / genetics
Substances
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Antiviral Agents
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Thymidine Kinase
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beta-Galactosidase
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Ganciclovir