Abstract
To investigate the role of the apoptosis-related genes, Bcl-2, Bax and Survivin genes were analyzed. For the Bax gene, abnormality was detected in 1 of 7 cervical and 1 of 6 endometrial cancer cell lines, 1 of 25 cervical cancer tissues and none of 17 endometrial cancer tissues using PCR-SSCP. In 4 cervical and 2 endometrial cancer cell lines, the ratio of Bcl-2 to Bax expression was higher than the control ratio using Western blotting. Survivin mRNA was detectable in all cell lines and all cancer tissues. The data suggested that these apoptosis-related genes may play important roles in the pathway of carcinogenesis of human uterine cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / genetics*
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DNA, Neoplasm / genetics
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Endometrial Neoplasms / genetics*
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Endometrial Neoplasms / pathology
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Female
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Gene Expression Regulation, Neoplastic
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Genes, bcl-2*
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Humans
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins*
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Neoplasm Proteins
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational
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Protein Biosynthesis
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Proteins / genetics*
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis
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RNA, Messenger / biosynthesis
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RNA, Neoplasm / biosynthesis
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Survivin
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Uterine Cervical Neoplasms / genetics*
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bcl-2-Associated X Protein
Substances
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BAX protein, human
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BIRC5 protein, human
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DNA, Neoplasm
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins
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Neoplasm Proteins
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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RNA, Neoplasm
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Survivin
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bcl-2-Associated X Protein