Abstract
In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC). The three genes identified encode phospholipase C and two potential inositol polyphosphate kinases. Together, these constitute a signaling pathway from phosphatidylinositol 4, 5-bisphosphate to inositol hexakisphosphate (IP6). The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biological Transport
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Genes, Fungal
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Genetic Complementation Test
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Inositol Phosphates / metabolism
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Mutation
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Nuclear Envelope / metabolism*
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Nuclear Pore Complex Proteins
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Phytic Acid / metabolism
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RNA, Fungal / metabolism
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RNA, Messenger / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Signal Transduction
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Type C Phospholipases / metabolism*
Substances
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Carrier Proteins
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GLE1 protein, S cerevisiae
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Inositol Phosphates
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Nuclear Pore Complex Proteins
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RNA, Fungal
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Phytic Acid
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inositol 4,5-bisphosphate
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IPK1 protein, S cerevisiae
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Phosphotransferases (Alcohol Group Acceptor)
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inositol pentakisphosphate kinase
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Type C Phospholipases