Further characterization of the human clear cell sarcoma cell line HS-MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF-1 transcript

J Pathol. 1999 Apr;187(5):594-7. doi: 10.1002/(SICI)1096-9896(199904)187:5<594::AID-PATH277>3.0.CO;2-H.

Abstract

Only a small number of clear cell sarcoma (CCS) cell lines have been reported, including the cell line HS-MM. In the present study, this cell line, maintained for more than 4 years since establishment, was further characterized by cytogenetic studies, fluorescence in situ hybridization (FISH) analysis, and reverse transcriptase-polymerase chain reaction (RT-PCR). HS-MM cells both in vitro and in vivo exhibited pseudodiploid karyotypes with the specific t(12;22)(q13;q12) translocation. The translocation between chromosomes 12 and 22 was confirmed by FISH analysis and the hybrid EWS/ATF-1 transcript induced by this translocation was detected by RT-PCR. The HS-MM cell line will be useful for further studies of CCS.

MeSH terms

  • Chromosome Aberrations
  • Chromosomes, Human, Pair 12*
  • Chromosomes, Human, Pair 22*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Oncogene Proteins, Fusion / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Clear Cell / genetics*
  • Sarcoma, Clear Cell / pathology
  • Transcription Factors / genetics
  • Translocation, Genetic*
  • Tumor Cells, Cultured*

Substances

  • EWS-ATF1 fusion protein, human
  • Oncogene Proteins, Fusion
  • Transcription Factors