Mutated cytochrome b as a determinant of a new monoclonal antibody (H8.98) on renal carcinoma cell lines recognized by a Vgamma3Vdelta1(+) T-cell clone

Int J Cancer. 1999 Aug 12;82(4):562-8. doi: 10.1002/(sici)1097-0215(19990812)82:4<562::aid-ijc15>3.0.co;2-w.

Abstract

We generated a monoclonal antibody (MAb), H8.98, that recognizes an antigen shared by 50% of examined renal carcinoma cell (RCC) lines and is susceptible to lysis by a Vgamma3Vdelta1(+) T-cell clone derived from RCC tumor-infiltrating lymphocytes. H8.98 inhibited Vgamma3Vdelta1(+ )T-cell clone-mediated lysis of RCC lines. It did not stain normal kidney lines, melanomas, fibroblasts, Burkitt's lymphoma or Epstein-Barr virus-transformed B-cell lines but it did stain 2 of 4 tested breast cancer lines. Through screening of a renal carcinoma cDNA library using H8.98, we isolated a cDNA clone which, upon sequencing, was found to be cytochrome b with 2 point mutations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / immunology
  • COS Cells
  • Carcinoma, Renal Cell / genetics
  • Carcinoma, Renal Cell / immunology*
  • Cytochrome b Group / genetics*
  • Cytochrome b Group / immunology
  • Humans
  • Immunohistochemistry
  • Kidney Neoplasms / genetics
  • Kidney Neoplasms / immunology*
  • Molecular Sequence Data
  • Point Mutation / genetics*
  • T-Lymphocytes / immunology
  • Transcription, Genetic*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • Cytochrome b Group