Abstract
We describe the epitope specificity of a panel of ten demyelinating monoclonal antibodies (mAb) that recognise the extracellular immunoglobulin-like domain of human myelin oligodendrocyte glycoprotein (hMOG(lgd)). All the mAbs bind to the surface of MOG-transfected fibroblasts as assessed in vitro by FACS and immunocytochemistry but failed to recognise overlapping 15-mer MOG peptides when assessed by ELISA. However, increasing peptide length to 25 amino acids revealed that four mAbs recognised epitopes within the amino acid sequence 63-100 of human MOG. In contrast, a non-demyelinating MOG-specific mAb recognised MOG by both ELISA and Western blotting but failed to stain MOG transfected fibroblasts. These observations suggest that assays based on the use of MOG-transfected cell lines will differentiate between pathogenic and non-pathogenic MOG-specific antibody responses in experimental models and human diseases of the nervous system.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibodies, Monoclonal / immunology*
-
Antibody Specificity
-
Autoantibodies / immunology
-
Autoimmune Diseases / immunology
-
Blotting, Western
-
Cells, Cultured
-
Demyelinating Diseases / immunology*
-
Enzyme-Linked Immunosorbent Assay
-
Epitopes / immunology*
-
Fibroblasts / cytology
-
Fluorescent Antibody Technique
-
Gene Expression Regulation / immunology
-
Humans
-
Immunoglobulin G / analysis
-
Immunoglobulin G / immunology
-
Multiple Sclerosis / immunology
-
Myelin Proteins
-
Myelin-Associated Glycoprotein / analysis
-
Myelin-Associated Glycoprotein / genetics
-
Myelin-Associated Glycoprotein / immunology*
-
Myelin-Oligodendrocyte Glycoprotein
-
Oligodendroglia / chemistry*
-
Oligodendroglia / immunology
-
Recombinant Proteins / genetics
-
Recombinant Proteins / immunology
-
Transfection
Substances
-
Antibodies, Monoclonal
-
Autoantibodies
-
Epitopes
-
Immunoglobulin G
-
MOG protein, human
-
Myelin Proteins
-
Myelin-Associated Glycoprotein
-
Myelin-Oligodendrocyte Glycoprotein
-
Recombinant Proteins