Abstract
Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.73, b = 49.35, c = 92.15 A and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1. 78 A resolution using synchrotron radiation. A molecular-replacement solution was obtained using the human Reg/lithostathine structure and the AMoRe software.
MeSH terms
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Acute-Phase Proteins / chemistry*
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Acute-Phase Proteins / genetics
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Acute-Phase Proteins / isolation & purification*
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Animals
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Antigens, Neoplasm*
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Biomarkers, Tumor / chemistry*
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / isolation & purification*
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Carcinoma, Hepatocellular / chemistry*
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Crystallization
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Crystallography, X-Ray
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Female
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Humans
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Lectins / chemistry*
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Lectins / genetics
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Lectins / isolation & purification*
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Lectins, C-Type*
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Liver Neoplasms / chemistry*
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Mice
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Mice, Transgenic
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Milk / chemistry
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Pancreatitis-Associated Proteins
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Proteins*
Substances
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Acute-Phase Proteins
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Antigens, Neoplasm
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Biomarkers, Tumor
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Lectins
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Lectins, C-Type
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Pancreatitis-Associated Proteins
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Proteins
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REG3A protein, human
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Reg3b protein, mouse