Abstract
The cloning of full length cDNA for the translocon-associated protein alpha subunit, previously called signal sequence receptor alpha, is reported as a result of differential display experiments in search of genes induced by granulocyte-macrophage colony-stimulating factor. Its messenger RNA was more abundant in growing cells than in either factor-deprived cells or quiescent cells and comprised four species, each having microheterogeneity, as a result of complex alternative polyadenylation apparently dependent on arrays of non-canonical polyadenylation signals. Radiation hybrid mapping of the gene showed that the gene is on the short arm of chromosome 6.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Calcium-Binding Proteins / genetics*
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 6 / genetics
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Cloning, Molecular
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DNA, Complementary / genetics
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Gene Expression
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
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Humans
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Membrane Glycoproteins*
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Molecular Sequence Data
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Poly A / chemistry
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RNA, Messenger / biosynthesis*
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RNA, Messenger / chemistry
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RNA, Messenger / genetics*
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Peptide / genetics*
Substances
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Calcium-Binding Proteins
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DNA, Complementary
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Membrane Glycoproteins
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear
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Receptors, Peptide
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signal sequence receptor
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Poly A
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Granulocyte-Macrophage Colony-Stimulating Factor