Abstract
Patients who receive peripheral blood stem cell transplants are at risk of developing cancer recurrence due to the presence of malignant cells in the transplants. We investigated a sensitive method to detect malignant cells in the peripheral blood and peripheral blood stem cells of patients with testicular cancer using nested, reverse transcription-polymerase chain reaction (RT-PCR) to measure alpha-fetoprotein gene expression. Using this technique, a single cancer cell could be detected in 10(6) peripheral blood mononuclear cells. This is the first report of an attempt to detect circulating malignant cells in the peripheral blood of patients with testicular cancer by nested RT-PCR.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Actins / genetics
-
Adult
-
Carcinoma, Embryonal / blood
-
Carcinoma, Embryonal / genetics
-
Carcinoma, Embryonal / pathology
-
Carcinoma, Hepatocellular / genetics
-
Endodermal Sinus Tumor / blood
-
Endodermal Sinus Tumor / genetics
-
Endodermal Sinus Tumor / pathology
-
Gene Expression
-
Germinoma / blood
-
Germinoma / genetics
-
Germinoma / pathology
-
Hematopoietic Stem Cell Transplantation
-
Hematopoietic Stem Cells / chemistry
-
Hematopoietic Stem Cells / pathology
-
Humans
-
Male
-
Middle Aged
-
Monocytes / chemistry
-
Neoplasms, Germ Cell and Embryonal / blood
-
Neoplasms, Germ Cell and Embryonal / genetics
-
Neoplasms, Germ Cell and Embryonal / pathology*
-
Neoplastic Cells, Circulating / pathology*
-
RNA, Messenger / metabolism
-
RNA, Neoplasm / analysis
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sensitivity and Specificity
-
Teratoma / blood
-
Teratoma / genetics
-
Teratoma / pathology
-
Testicular Neoplasms / blood
-
Testicular Neoplasms / genetics
-
Testicular Neoplasms / pathology*
-
Tumor Cells, Cultured
-
alpha-Fetoproteins / genetics
Substances
-
Actins
-
RNA, Messenger
-
RNA, Neoplasm
-
alpha-Fetoproteins