Abstract
Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte-derived PR3 in CF-associated airway inflammation.
Publication types
-
Clinical Trial
-
Controlled Clinical Trial
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adolescent
-
Adult
-
Anti-Bacterial Agents / pharmacology
-
Anti-Bacterial Agents / therapeutic use
-
Bacterial Infections / complications
-
Bacterial Infections / drug therapy
-
Bacterial Infections / enzymology*
-
Child
-
Child, Preschool
-
Cystic Fibrosis / complications
-
Cystic Fibrosis / drug therapy
-
Cystic Fibrosis / enzymology*
-
Female
-
Humans
-
Infant
-
Male
-
Monocytes / enzymology*
-
Myeloblastin
-
Neutrophils / enzymology*
-
Peroxidase / genetics
-
RNA, Messenger / analysis*
-
RNA, Messenger / drug effects
-
Reference Values
-
Serine Endopeptidases / drug effects
-
Serine Endopeptidases / genetics*
-
Serine Endopeptidases / metabolism
-
Sputum / chemistry
-
Up-Regulation
Substances
-
Anti-Bacterial Agents
-
RNA, Messenger
-
Peroxidase
-
Serine Endopeptidases
-
Myeloblastin