Abstract
Expression of angiogenesis-associated genes was compared in 32 primary non-small cell lung carcinoma samples (14 adenocarcinomas, 17 squamous cell carcinomas, and 1 large cell carcinoma) and paired adjacent noncancerous lung tissues using a multiprobe RNase protection assay. Levels of Tie2, angiopoietin (Ang)-1, vascular endothelial growth factor (VEGF), and CD31 mRNAs were higher in cancers than in adjacent noncancerous tissues, in contrast to the fms-like tyrosine kinase (Flt)-1, Flt-4, Tie1, thrombin receptor, endoglin, and VEGF-C, for which no differences were evident. Overexpression did not seem to differ with histological type and pathological stage. Significant positive correlations were found between mRNA expression of Ang-1 and those of Tie2 and CD31, and that of VEGF and those of Flt-1 and CD31. These findings suggest that Ang-1 and VEGF are important angiogenic factors in human non-small cell lung carcinomas.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Angiopoietin-1
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Biomarkers
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Carcinoma, Non-Small-Cell Lung / blood supply
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Carcinoma, Non-Small-Cell Lung / enzymology
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Carcinoma, Non-Small-Cell Lung / metabolism*
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Endothelial Growth Factors / biosynthesis*
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Female
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Humans
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Ligands
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Lung Neoplasms / blood supply
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Lung Neoplasms / enzymology
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Lung Neoplasms / metabolism*
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Lymphokines / biosynthesis*
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Male
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Membrane Glycoproteins / biosynthesis*
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Middle Aged
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Neovascularization, Pathologic / genetics
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Neovascularization, Pathologic / metabolism
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Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis*
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Receptor Protein-Tyrosine Kinases / biosynthesis*
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Receptor, TIE-2
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Ribonucleases / metabolism
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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ANGPT1 protein, human
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Angiopoietin-1
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Biomarkers
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Endothelial Growth Factors
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Ligands
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Lymphokines
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Membrane Glycoproteins
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Platelet Endothelial Cell Adhesion Molecule-1
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Receptor Protein-Tyrosine Kinases
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Receptor, TIE-2
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Ribonucleases