Enhanced expression of Tie2, its ligand angiopoietin-1, vascular endothelial growth factor, and CD31 in human non-small cell lung carcinomas

Clin Cancer Res. 1999 Sep;5(9):2506-10.

Abstract

Expression of angiogenesis-associated genes was compared in 32 primary non-small cell lung carcinoma samples (14 adenocarcinomas, 17 squamous cell carcinomas, and 1 large cell carcinoma) and paired adjacent noncancerous lung tissues using a multiprobe RNase protection assay. Levels of Tie2, angiopoietin (Ang)-1, vascular endothelial growth factor (VEGF), and CD31 mRNAs were higher in cancers than in adjacent noncancerous tissues, in contrast to the fms-like tyrosine kinase (Flt)-1, Flt-4, Tie1, thrombin receptor, endoglin, and VEGF-C, for which no differences were evident. Overexpression did not seem to differ with histological type and pathological stage. Significant positive correlations were found between mRNA expression of Ang-1 and those of Tie2 and CD31, and that of VEGF and those of Flt-1 and CD31. These findings suggest that Ang-1 and VEGF are important angiogenic factors in human non-small cell lung carcinomas.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Angiopoietin-1
  • Biomarkers
  • Carcinoma, Non-Small-Cell Lung / blood supply
  • Carcinoma, Non-Small-Cell Lung / enzymology
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Endothelial Growth Factors / biosynthesis*
  • Female
  • Humans
  • Ligands
  • Lung Neoplasms / blood supply
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / metabolism*
  • Lymphokines / biosynthesis*
  • Male
  • Membrane Glycoproteins / biosynthesis*
  • Middle Aged
  • Neovascularization, Pathologic / genetics
  • Neovascularization, Pathologic / metabolism
  • Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis*
  • Receptor Protein-Tyrosine Kinases / biosynthesis*
  • Receptor, TIE-2
  • Ribonucleases / metabolism
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • ANGPT1 protein, human
  • Angiopoietin-1
  • Biomarkers
  • Endothelial Growth Factors
  • Ligands
  • Lymphokines
  • Membrane Glycoproteins
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Receptor Protein-Tyrosine Kinases
  • Receptor, TIE-2
  • Ribonucleases