Expression of cell-homologous genes of human herpesvirus-8 in human immunodeficiency virus-negative lymphoproliferative diseases

Blood. 1999 Oct 15;94(8):2931-3.

Abstract

Human herpesvirus-8 (HHV-8) genome encodes for genes homologous to human cellular genes such as interleukin-6 (IL-6), Cyclin-D, BCL-2, and IL-8 receptor (G-protein-coupled receptor [GCR]). We used reverse transcriptase-polymerase chain reaction to study the expression of these viral genes in lymphoproliferative disorders associated with HHV-8 infection. None of these genes was expressed in 1 case of benign, localized Castleman's disease (CD), and only viral IL-6 and viral Cyclin-D were transcribed in 2 cases of benign lymphadenopathies with giant germinal center hyperplasia and increased vascularity. In contrast, all 4 genes were transcribed in 1 case of multicentric CD of plasma cell type with aggressive clinical course and in 1 primary effusion lymphoma cell line. Our study provides the evidence that various HHV-8 genes, homologous to cellular genes involved in control of proliferation and apoptosis, may be differently expressed in different lymphoid disorders in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics
  • Apoptosis
  • Castleman Disease / classification
  • Castleman Disease / genetics
  • Castleman Disease / metabolism
  • Castleman Disease / pathology
  • Castleman Disease / virology*
  • Cell Division
  • Cyclin D
  • Cyclins / biosynthesis
  • Cyclins / genetics
  • Genes, bcl-2
  • HIV / isolation & purification*
  • Herpesvirus 8, Human / genetics*
  • Herpesvirus 8, Human / isolation & purification
  • Humans
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / genetics
  • Lymphatic Diseases / genetics
  • Lymphatic Diseases / metabolism
  • Lymphatic Diseases / pathology
  • Lymphatic Diseases / virology
  • Lymphoid Tissue / metabolism*
  • Lymphoid Tissue / virology
  • Lymphoproliferative Disorders / genetics
  • Lymphoproliferative Disorders / metabolism
  • Lymphoproliferative Disorders / virology*
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin-8A
  • Reverse Transcriptase Polymerase Chain Reaction
  • Species Specificity
  • Transcription, Genetic
  • Viral Proteins / biosynthesis*
  • Viral Proteins / genetics
  • Virus Latency

Substances

  • Antigens, CD
  • Cyclin D
  • Cyclins
  • Interleukin-6
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • Viral Proteins