Abstract
Defensins contribute to host defense by disrupting the cytoplasmic membrane of microorganisms. This report shows that human beta-defensins are also chemotactic for immature dendritic cells and memory T cells. Human beta-defensin was selectively chemotactic for cells stably transfected to express human CCR6, a chemokine receptor preferentially expressed by immature dendritic cells and memory T cells. The beta-defensin-induced chemotaxis was sensitive to pertussis toxin and inhibited by antibodies to CCR6. The binding of iodinated LARC, the chemokine ligand for CCR6, to CCR6-transfected cells was competitively displaced by beta-defensin. Thus, beta-defensins may promote adaptive immune responses by recruiting dendritic and T cells to the site of microbial invasion through interaction with CCR6.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies / immunology
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Binding, Competitive
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Cell Line
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Chemokine CCL20
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Chemokines, CC / metabolism
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Chemokines, CC / pharmacology
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Chemotaxis
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Chemotaxis, Leukocyte
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Defensins
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Dendritic Cells / immunology*
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Humans
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Immunity, Active*
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Immunity, Innate*
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Immunologic Memory
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Macrophage Inflammatory Proteins*
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Pertussis Toxin
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Proteins / pharmacology
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Proteins / physiology*
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Receptors, CCR6
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Receptors, Chemokine / genetics
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Receptors, Chemokine / metabolism*
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Recombinant Proteins / pharmacology
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T-Lymphocyte Subsets / immunology*
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Transfection
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Virulence Factors, Bordetella / pharmacology
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beta-Defensins*
Substances
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Antibodies
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CCL20 protein, human
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CCR6 protein, human
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Chemokine CCL20
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Chemokines, CC
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DEFB4A protein, human
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Defensins
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Macrophage Inflammatory Proteins
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Proteins
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Receptors, CCR6
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Receptors, Chemokine
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Recombinant Proteins
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Virulence Factors, Bordetella
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beta-Defensins
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Pertussis Toxin