Abstract
A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterization of a novel bio gene: bio2, encoding biotin synthase from Schizosaccharomyces pombe.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biotin / biosynthesis
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Biotin / genetics
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Cloning, Molecular*
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Genetic Complementation Test
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Molecular Sequence Data
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Mutagenesis
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces / genetics*
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Sulfurtransferases / genetics*
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Sulfurtransferases / metabolism
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Transformation, Genetic
Substances
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Biotin
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Sulfurtransferases
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biotin synthetase