Abstract
Epsin (epsin 1) is an interacting partner for the EH domain-containing region of Eps15 and has been implicated in conjunction with Eps15 in clathrin-mediated endocytosis. We report here the characterization of a similar protein (epsin 2), which we have cloned from human and rat brain libraries. Epsin 1 and 2 are most similar in their NH(2)-terminal region, which represents a module (epsin NH(2) terminal homology domain, ENTH domain) found in a variety of other proteins of the data base. The multiple DPW motifs, typical of the central region of epsin 1, are only partially conserved in epsin 2. Both proteins, however, interact through this central region with the clathrin adaptor AP-2. In addition, we show here that both epsin 1 and 2 interact with clathrin. The three NPF motifs of the COOH-terminal region of epsin 1 are conserved in the corresponding region of epsin 2, consistent with the binding of both proteins to Eps15. Epsin 2, like epsin 1, is enriched in brain, is present in a brain-derived clathrin-coated vesicle fraction, is concentrated in the peri-Golgi region and at the cell periphery of transfected cells, and partially colocalizes with clathrin. High overexpression of green fluorescent protein-epsin 2 mislocalizes components of the clathrin coat and inhibits clathrin-mediated endocytosis. The epsins define a new protein family implicated in membrane dynamics at the cell surface.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Amino Acid Sequence
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Animals
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CHO Cells
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Calcium-Binding Proteins / metabolism
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Clathrin / metabolism*
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Coated Vesicles / metabolism
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Cricetinae
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Fluorescent Antibody Technique
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Gene Expression
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Humans
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Intracellular Signaling Peptides and Proteins
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Luciferases / genetics
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Luciferases / metabolism
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Male
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Neuropeptides / chemistry
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Neuropeptides / genetics*
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Neuropeptides / metabolism
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Phosphoproteins / metabolism
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Phylogeny
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Protein Binding
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Protein Structure, Tertiary
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Rats
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
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Vesicular Transport Proteins*
Substances
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Calcium-Binding Proteins
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Carrier Proteins
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Clathrin
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DNA, Complementary
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EPN2 protein, human
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EPS15 protein, human
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Neuropeptides
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Phosphoproteins
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Recombinant Fusion Proteins
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Vesicular Transport Proteins
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epsin
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Luciferases
Associated data
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GENBANK/AF062084
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GENBANK/AF062085