Abstract
We have established a highly-metastatic cell line (designated as HNOS) and a non-metastatic cell line (designated as SAT) derived from human oral cavity squamous cell carcinoma (SCC). Both lines were transplantable in nude mice. The invasive activity through Matrigel-coated membrane of HNOS cells was also higher than that of SAT cells. mRNA of TIMP-1 was expressed in SAT cells but not in HNOS cells. Metastatic and invasive abilities were suppressed by the overexpression of TIMP-1 in HNOS cells. These results suggest that TIMP-1 may have an important role in inhibiting invasion and metastasis of human oral cavity SCC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carcinoma, Squamous Cell / prevention & control*
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Carcinoma, Squamous Cell / secondary
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DNA, Complementary / genetics
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Humans
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Matrix Metalloproteinase 1 / biosynthesis
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Matrix Metalloproteinase 1 / genetics
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Mouth Neoplasms / pathology
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Mouth Neoplasms / prevention & control*
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Neoplasm Invasiveness / prevention & control
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Neoplasm Metastasis / prevention & control
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Neoplasm Transplantation
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Tissue Inhibitor of Metalloproteinase-1 / biosynthesis
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Tissue Inhibitor of Metalloproteinase-1 / genetics
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Tissue Inhibitor of Metalloproteinase-1 / therapeutic use*
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Transfection
Substances
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DNA, Complementary
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Tissue Inhibitor of Metalloproteinase-1
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Matrix Metalloproteinase 1