Abstract
We have used human retinal pigment epithelial (HRPE) cultures to investigate the primary cellular responses of retinal resident cells to intracellular Toxoplasma gondii replication. At 4 days postinoculation, when all of the cells were infected, the secretion of interleukin 1beta (IL-1beta), IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and intercellular adhesion molecule 1 (ICAM-1) was augmented by 23-, 10-, 8-, and 5-fold, respectively, over the control. Northern and reverse transcriptase PCR analyses showed significant upregulation of steady-state levels of mRNA for IL-1beta, IL-6, GM-CSF, and ICAM-1. The secretion of these molecules by HRPE cells may play a critical immunoregulatory role in the pathophysiological processes associated with T. gondii-induced retinochoroiditis.
MeSH terms
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Animals
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Cell Line
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Chorioretinitis / etiology
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Chorioretinitis / genetics
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Chorioretinitis / immunology
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
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Humans
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Intercellular Adhesion Molecule-1 / biosynthesis*
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Intercellular Adhesion Molecule-1 / genetics*
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Interleukin-1 / genetics*
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Interleukin-1 / metabolism*
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Interleukin-6 / genetics*
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Interleukin-6 / metabolism*
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Pigment Epithelium of Eye / immunology*
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Pigment Epithelium of Eye / parasitology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Toxoplasma / immunology
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Toxoplasma / pathogenicity*
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Toxoplasmosis, Ocular / genetics
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Toxoplasmosis, Ocular / immunology
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Up-Regulation
Substances
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Interleukin-1
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Interleukin-6
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RNA, Messenger
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Intercellular Adhesion Molecule-1
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Granulocyte-Macrophage Colony-Stimulating Factor