Abstract
In this study we aimed to test the hypothesis that leukaemias with oligoclonal Vdelta2-Ddelta3 rearrangements are clonal at the IgH and TCRG gene status and that the oligoclonality is a poor prognostic marker. In ten leukaemias the individual Vdelta2-Ddelta3 rearrangements characterised different populations as deduced from single cell analysis and/or from densitometric differences of PCR products. Five of these leukaemias were clonal by the IgH and/or TCRG gene status. We therefore conclude that ALL is a clonal disease despite the presence of heterogeneous TCRD rearrangements. Our clinical data show that oligoclonality at the TCRD level does not represent an adverse prognostic factor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aneuploidy
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Cell Transformation, Neoplastic / genetics
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Child
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Child, Preschool
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Clone Cells / chemistry
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Clone Cells / immunology
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Female
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Gene Rearrangement, delta-Chain T-Cell Antigen Receptor*
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Genes, Immunoglobulin*
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Humans
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Immunoglobulin Heavy Chains / genetics*
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Infant
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Karyotyping
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Male
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Neoplastic Stem Cells / chemistry
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Neoplastic Stem Cells / immunology
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / immunology
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / mortality
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Prognosis
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Receptors, Antigen, T-Cell, gamma-delta / genetics*
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Risk
Substances
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Immunoglobulin Heavy Chains
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Receptors, Antigen, T-Cell, gamma-delta