Heterogeneous TCR delta Vdelta2-Ddelta3 rearrangements and their relation to IgH and TCR gamma gene status in childhood B cell precursor leukaemias

Leuk Res. 1999 Dec;23(12):1089-96. doi: 10.1016/s0145-2126(99)00121-6.

Abstract

In this study we aimed to test the hypothesis that leukaemias with oligoclonal Vdelta2-Ddelta3 rearrangements are clonal at the IgH and TCRG gene status and that the oligoclonality is a poor prognostic marker. In ten leukaemias the individual Vdelta2-Ddelta3 rearrangements characterised different populations as deduced from single cell analysis and/or from densitometric differences of PCR products. Five of these leukaemias were clonal by the IgH and/or TCRG gene status. We therefore conclude that ALL is a clonal disease despite the presence of heterogeneous TCRD rearrangements. Our clinical data show that oligoclonality at the TCRD level does not represent an adverse prognostic factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aneuploidy
  • Cell Transformation, Neoplastic / genetics
  • Child
  • Child, Preschool
  • Clone Cells / chemistry
  • Clone Cells / immunology
  • Female
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor*
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Infant
  • Karyotyping
  • Male
  • Neoplastic Stem Cells / chemistry
  • Neoplastic Stem Cells / immunology
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / immunology
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / mortality
  • Prognosis
  • Receptors, Antigen, T-Cell, gamma-delta / genetics*
  • Risk

Substances

  • Immunoglobulin Heavy Chains
  • Receptors, Antigen, T-Cell, gamma-delta