Abstract
Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment.
Copyright 2000 Wiley-Liss, Inc.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Blotting, Southern
-
Bone Marrow Cells / cytology
-
Bone Marrow Cells / metabolism
-
Cells, Cultured
-
Child
-
Chromosomes, Human, Pair 22 / genetics
-
Clone Cells
-
Drug Administration Schedule
-
Female
-
Follow-Up Studies
-
Gene Rearrangement*
-
Genes, abl / genetics*
-
Humans
-
Interferon Type I / administration & dosage*
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
-
Male
-
Meta-Analysis as Topic
-
Middle Aged
-
Philadelphia Chromosome
-
RNA, Messenger / metabolism
-
Recombinant Proteins
-
Restriction Mapping
-
Reverse Transcriptase Polymerase Chain Reaction
-
Time
-
Translocation, Genetic / genetics*
Substances
-
Interferon Type I
-
RNA, Messenger
-
Recombinant Proteins