Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Am J Hematol. 2000 Feb;63(2):85-9. doi: 10.1002/(sici)1096-8652(200002)63:2<85::aid-ajh5>3.0.co;2-l.

Abstract

Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blotting, Southern
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Cells, Cultured
  • Child
  • Chromosomes, Human, Pair 22 / genetics
  • Clone Cells
  • Drug Administration Schedule
  • Female
  • Follow-Up Studies
  • Gene Rearrangement*
  • Genes, abl / genetics*
  • Humans
  • Interferon Type I / administration & dosage*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Male
  • Meta-Analysis as Topic
  • Middle Aged
  • Philadelphia Chromosome
  • RNA, Messenger / metabolism
  • Recombinant Proteins
  • Restriction Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time
  • Translocation, Genetic / genetics*

Substances

  • Interferon Type I
  • RNA, Messenger
  • Recombinant Proteins