Cloning, characterization and genomic organization of LCC-1 (scya16), a novel human CC chemokine expressed in liver

Cytokine. 2000 Feb;12(2):101-9. doi: 10.1006/cyto.1999.0548.

Abstract

By homology search of expressed sequence tags (EST) in GenBank a novel member of the CC chemokine family was identified. The full-length sequence of this liver-specific CC chemokine (LCC-1) predicted a mature protein of 97 amino acids with 31-48% identity to other CC chemokines. There was a characteristic amino acid C-term extension when aligned with other chemokines. Northern blot analysis from a panel of human tissues revealed that LCC-1 mRNA expression is restricted to adult and fetal liver. Different polyadenylation results in two mRNA species of 1.5 kb and 0.5 kb in size. LCC-1 is constitutively expressed in human HepG2 hepatoma cells and is induced by hypoxic exposure. The promoter region of the LCC-1 gene contains potential HIF-1 binding sites. The EST for LCC-1 has been previously mapped to the CC chemokine cluster on human chromosome 17q11.2. The organization of the LCC-1 gene (scya16) into three exons interrupted by two introns is identical to that found for other members of the CC chemokine family.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites / genetics
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / immunology
  • Cell Hypoxia / genetics
  • Cell Hypoxia / immunology
  • Chemokines, CC / genetics*
  • Chromosomes, Human, Pair 17 / genetics
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / metabolism
  • Expressed Sequence Tags
  • Gene Expression
  • Humans
  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Liver / immunology*
  • Liver Neoplasms / genetics
  • Liver Neoplasms / immunology
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • CCL16 protein, human
  • Chemokines, CC
  • DNA-Binding Proteins
  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Nuclear Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/AF039954
  • GENBANK/AF039955