Abstract
By homology search of expressed sequence tags (EST) in GenBank a novel member of the CC chemokine family was identified. The full-length sequence of this liver-specific CC chemokine (LCC-1) predicted a mature protein of 97 amino acids with 31-48% identity to other CC chemokines. There was a characteristic amino acid C-term extension when aligned with other chemokines. Northern blot analysis from a panel of human tissues revealed that LCC-1 mRNA expression is restricted to adult and fetal liver. Different polyadenylation results in two mRNA species of 1.5 kb and 0.5 kb in size. LCC-1 is constitutively expressed in human HepG2 hepatoma cells and is induced by hypoxic exposure. The promoter region of the LCC-1 gene contains potential HIF-1 binding sites. The EST for LCC-1 has been previously mapped to the CC chemokine cluster on human chromosome 17q11.2. The organization of the LCC-1 gene (scya16) into three exons interrupted by two introns is identical to that found for other members of the CC chemokine family.
Copyright 2000 Academic Press.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adult
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Amino Acid Sequence
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Base Sequence
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Binding Sites / genetics
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / immunology
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Cell Hypoxia / genetics
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Cell Hypoxia / immunology
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Chemokines, CC / genetics*
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Chromosomes, Human, Pair 17 / genetics
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Cloning, Molecular
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DNA / genetics
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DNA-Binding Proteins / metabolism
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Expressed Sequence Tags
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Gene Expression
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Humans
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Liver / immunology*
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Liver Neoplasms / genetics
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Liver Neoplasms / immunology
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Molecular Sequence Data
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Molecular Weight
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Sequence Homology, Amino Acid
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Transcription Factors*
Substances
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CCL16 protein, human
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Chemokines, CC
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DNA-Binding Proteins
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HIF1A protein, human
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Nuclear Proteins
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RNA, Messenger
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RNA, Neoplasm
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Transcription Factors
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DNA
Associated data
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GENBANK/AF039954
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GENBANK/AF039955