Identification of viral macrophage inflammatory protein (vMIP)-II as a ligand for GPR5/XCR1

Biochem Biophys Res Commun. 2000 Feb 24;268(3):938-41. doi: 10.1006/bbrc.2000.2235.

Abstract

Lymphotactin is unique among chemokines in that it contains only two of four conserved cysteines and may possess a structure less constrained than other chemokines. The viral chemokine vMIP-II, which presumably has a structure similar to that of CC chemokines has been shown to inhibit many chemokine receptors, but its activity at GPR5/XCR1 has not been described. Interestingly, vMIP-II (but not vMIP-I) was found to be a potent antagonist of lymphotactin activity at GPR5/XCR1, extending the range of chemokine classes that this viral protein is known to inhibit to include the C class chemokine. In addition, we have extended previous analyses of GPR5/XCR1 expression and show that this receptor is expressed in leukocyte cells previously shown to be responsive to lymphotactin.

MeSH terms

  • Animals
  • Base Sequence
  • Calcium / metabolism
  • Cell Line
  • Chemokines / metabolism*
  • Chemokines, C*
  • Cloning, Molecular
  • DNA Primers / genetics
  • Gene Expression
  • Humans
  • Ligands
  • Lymphokines / metabolism
  • Macrophage Inflammatory Proteins / metabolism*
  • Membrane Proteins*
  • Mice
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Receptors, G-Protein-Coupled*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sialoglycoproteins / metabolism
  • Tissue Distribution
  • Viral Proteins / metabolism*

Substances

  • Chemokines
  • Chemokines, C
  • DNA Primers
  • Ligands
  • Lymphokines
  • Macrophage Inflammatory Proteins
  • Membrane Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Recombinant Proteins
  • Sialoglycoproteins
  • Viral Proteins
  • XCL1 protein, human
  • XCR1 protein, human
  • Xcl1 protein, mouse
  • lymphotactin
  • vMIP-II
  • Calcium