Abstract
Lymphotactin is unique among chemokines in that it contains only two of four conserved cysteines and may possess a structure less constrained than other chemokines. The viral chemokine vMIP-II, which presumably has a structure similar to that of CC chemokines has been shown to inhibit many chemokine receptors, but its activity at GPR5/XCR1 has not been described. Interestingly, vMIP-II (but not vMIP-I) was found to be a potent antagonist of lymphotactin activity at GPR5/XCR1, extending the range of chemokine classes that this viral protein is known to inhibit to include the C class chemokine. In addition, we have extended previous analyses of GPR5/XCR1 expression and show that this receptor is expressed in leukocyte cells previously shown to be responsive to lymphotactin.
Copyright 2000 Academic Press.
MeSH terms
-
Animals
-
Base Sequence
-
Calcium / metabolism
-
Cell Line
-
Chemokines / metabolism*
-
Chemokines, C*
-
Cloning, Molecular
-
DNA Primers / genetics
-
Gene Expression
-
Humans
-
Ligands
-
Lymphokines / metabolism
-
Macrophage Inflammatory Proteins / metabolism*
-
Membrane Proteins*
-
Mice
-
Receptors, Cell Surface / genetics
-
Receptors, Cell Surface / metabolism*
-
Receptors, G-Protein-Coupled*
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Sialoglycoproteins / metabolism
-
Tissue Distribution
-
Viral Proteins / metabolism*
Substances
-
Chemokines
-
Chemokines, C
-
DNA Primers
-
Ligands
-
Lymphokines
-
Macrophage Inflammatory Proteins
-
Membrane Proteins
-
Receptors, Cell Surface
-
Receptors, G-Protein-Coupled
-
Recombinant Proteins
-
Sialoglycoproteins
-
Viral Proteins
-
XCL1 protein, human
-
XCR1 protein, human
-
Xcl1 protein, mouse
-
lymphotactin
-
vMIP-II
-
Calcium