Differential expression of an estrogen receptor messenger RNA containing exon 1' sequences in MCF-7 breast cancer cell line stocks

Cancer Lett. 2000 Jan 1;148(1):87-93. doi: 10.1016/s0304-3835(99)00317-1.

Abstract

The presence of an exon 1' sequence in the estrogen receptor alpha (ERalpha) mRNA was detected in different stocks of ER-positive MCF-7 human breast cancer cells by reverse transcriptase polymerase chain reaction (RT-PCR) and ribonuclease protection analysis (RPA), but not by Northern blot analysis. This mRNA, however, was not detectable in ERalpha-positive ZR-75-1 or ERalpha-negative MDA-MB-231 breast cancer cells, suggesting that exon 1' ER mRNA is differentially expressed in some but not all ER-positive cell lines, and then, only at very low levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cloning, Molecular
  • Estrogen Receptor alpha
  • Exons / genetics*
  • Gene Expression*
  • Genetic Variation / genetics
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / genetics*
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Receptors, Estrogen / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic / genetics
  • Tumor Cells, Cultured

Substances

  • Estrogen Receptor alpha
  • RNA, Messenger
  • Receptors, Estrogen