Abstract
The presence of an exon 1' sequence in the estrogen receptor alpha (ERalpha) mRNA was detected in different stocks of ER-positive MCF-7 human breast cancer cells by reverse transcriptase polymerase chain reaction (RT-PCR) and ribonuclease protection analysis (RPA), but not by Northern blot analysis. This mRNA, however, was not detectable in ERalpha-positive ZR-75-1 or ERalpha-negative MDA-MB-231 breast cancer cells, suggesting that exon 1' ER mRNA is differentially expressed in some but not all ER-positive cell lines, and then, only at very low levels.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Blotting, Northern
-
Breast Neoplasms / genetics*
-
Breast Neoplasms / pathology
-
Cloning, Molecular
-
Estrogen Receptor alpha
-
Exons / genetics*
-
Gene Expression*
-
Genetic Variation / genetics
-
Humans
-
Molecular Sequence Data
-
Open Reading Frames / genetics
-
Promoter Regions, Genetic / genetics
-
RNA, Messenger / genetics*
-
RNA, Messenger / isolation & purification
-
RNA, Messenger / metabolism
-
Receptors, Estrogen / genetics*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Transcription, Genetic / genetics
-
Tumor Cells, Cultured
Substances
-
Estrogen Receptor alpha
-
RNA, Messenger
-
Receptors, Estrogen