Trichostatin A modulates expression of p21waf1/cip1, Bcl-xL, ID1, ID2, ID3, CRAB2, GATA-2, hsp86 and TFIID/TAFII31 mRNA in human lung adenocarcinoma cells

Biol Chem. 2000 Feb;381(2):107-12. doi: 10.1515/BC.2000.015.

Abstract

Lung adenocarcinoma cells treated for 16 h with trichostatin A (TSA), an inhibitor of histone deacetylases, and untreated cells were analyzed with respect to differential gene expression. Complex hybridization of cDNA arrays revealed repression of Bcl-xL, CRAB2 and TFIID/TAFII31 as well as induction of p21waf1/cip1, GATA-2, hsp86, ID1, ID2 and ID3 mRNA expression, which could be verified by Northern blotting. ID2 induction was further confirmed by Taqman realtime quantitative RT-PCR. The described alterations of gene expression due to TSA renders the lung adenocarcinoma cells susceptible to induction of apoptosis.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Blotting, Northern
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins / genetics
  • DNA, Complementary
  • DNA-Binding Proteins / genetics
  • Enzyme Inhibitors / pharmacology
  • GATA2 Transcription Factor
  • Gene Expression Regulation, Neoplastic / drug effects*
  • HSP90 Heat-Shock Proteins / genetics
  • Humans
  • Hydroxamic Acids / pharmacology*
  • Inhibitor of Differentiation Protein 1
  • Inhibitor of Differentiation Protein 2
  • Inhibitor of Differentiation Proteins
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Neoplasm Proteins / genetics*
  • Nucleic Acid Hybridization
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • RNA, Messenger / drug effects*
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / drug effects*
  • RNA, Neoplasm / metabolism
  • Receptors, Retinoic Acid / genetics
  • Repressor Proteins*
  • Reverse Transcriptase Polymerase Chain Reaction
  • TATA-Binding Protein Associated Factors*
  • Trans-Activators / genetics
  • Transcription Factor TFIID
  • Transcription Factors / genetics
  • Transcription Factors, TFII / genetics
  • Tumor Cells, Cultured
  • bcl-X Protein

Substances

  • BCL2L1 protein, human
  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Enzyme Inhibitors
  • GATA2 Transcription Factor
  • GATA2 protein, human
  • HSP90 Heat-Shock Proteins
  • Hspca protein, mouse
  • Hydroxamic Acids
  • ID1 protein, human
  • ID2 protein, human
  • Inhibitor of Differentiation Protein 1
  • Inhibitor of Differentiation Protein 2
  • Inhibitor of Differentiation Proteins
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Retinoic Acid
  • Repressor Proteins
  • TAF9 protein, human
  • TATA-Binding Protein Associated Factors
  • Trans-Activators
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • bcl-X Protein
  • retinoic acid binding protein II, cellular
  • ID3 protein, human
  • trichostatin A