Abstract
Lung adenocarcinoma cells treated for 16 h with trichostatin A (TSA), an inhibitor of histone deacetylases, and untreated cells were analyzed with respect to differential gene expression. Complex hybridization of cDNA arrays revealed repression of Bcl-xL, CRAB2 and TFIID/TAFII31 as well as induction of p21waf1/cip1, GATA-2, hsp86, ID1, ID2 and ID3 mRNA expression, which could be verified by Northern blotting. ID2 induction was further confirmed by Taqman realtime quantitative RT-PCR. The described alterations of gene expression due to TSA renders the lung adenocarcinoma cells susceptible to induction of apoptosis.
MeSH terms
-
Adenocarcinoma / genetics*
-
Adenocarcinoma / pathology
-
Blotting, Northern
-
Cyclin-Dependent Kinase Inhibitor p21
-
Cyclins / genetics
-
DNA, Complementary
-
DNA-Binding Proteins / genetics
-
Enzyme Inhibitors / pharmacology
-
GATA2 Transcription Factor
-
Gene Expression Regulation, Neoplastic / drug effects*
-
HSP90 Heat-Shock Proteins / genetics
-
Humans
-
Hydroxamic Acids / pharmacology*
-
Inhibitor of Differentiation Protein 1
-
Inhibitor of Differentiation Protein 2
-
Inhibitor of Differentiation Proteins
-
Lung Neoplasms / genetics*
-
Lung Neoplasms / pathology
-
Neoplasm Proteins / genetics*
-
Nucleic Acid Hybridization
-
Proto-Oncogene Proteins c-bcl-2 / genetics
-
RNA, Messenger / drug effects*
-
RNA, Messenger / metabolism
-
RNA, Neoplasm / drug effects*
-
RNA, Neoplasm / metabolism
-
Receptors, Retinoic Acid / genetics
-
Repressor Proteins*
-
Reverse Transcriptase Polymerase Chain Reaction
-
TATA-Binding Protein Associated Factors*
-
Trans-Activators / genetics
-
Transcription Factor TFIID
-
Transcription Factors / genetics
-
Transcription Factors, TFII / genetics
-
Tumor Cells, Cultured
-
bcl-X Protein
Substances
-
BCL2L1 protein, human
-
CDKN1A protein, human
-
Cyclin-Dependent Kinase Inhibitor p21
-
Cyclins
-
DNA, Complementary
-
DNA-Binding Proteins
-
Enzyme Inhibitors
-
GATA2 Transcription Factor
-
GATA2 protein, human
-
HSP90 Heat-Shock Proteins
-
Hspca protein, mouse
-
Hydroxamic Acids
-
ID1 protein, human
-
ID2 protein, human
-
Inhibitor of Differentiation Protein 1
-
Inhibitor of Differentiation Protein 2
-
Inhibitor of Differentiation Proteins
-
Neoplasm Proteins
-
Proto-Oncogene Proteins c-bcl-2
-
RNA, Messenger
-
RNA, Neoplasm
-
Receptors, Retinoic Acid
-
Repressor Proteins
-
TAF9 protein, human
-
TATA-Binding Protein Associated Factors
-
Trans-Activators
-
Transcription Factor TFIID
-
Transcription Factors
-
Transcription Factors, TFII
-
bcl-X Protein
-
retinoic acid binding protein II, cellular
-
ID3 protein, human
-
trichostatin A