Abstract
Electron cryomicroscopy of the clathrin coat and X-ray crystallography of parts of the clathrin heavy chain combine to give a detailed picture of the clathrin molecule, assembled as a cage. Recently determined domain structures of other components of the endocytic machinery, particularly the mu2 subunit and the alpha-appendage domain of the AP2 adaptor complex, provide important information on the sequence of recognition events involved in the dynamic process of clathrin coat assembly.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Adaptor Protein Complex 1*
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Adaptor Protein Complex 2
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Adaptor Protein Complex 3*
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Adaptor Protein Complex alpha Subunits
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Adaptor Protein Complex mu Subunits*
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Adaptor Proteins, Vesicular Transport
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Animals
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Clathrin / chemistry*
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Clathrin / physiology
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Clathrin / ultrastructure
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Coated Pits, Cell-Membrane / chemistry*
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Cryoelectron Microscopy
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Crystallography, X-Ray
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Endocytosis / physiology*
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Humans
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Membrane Proteins / chemistry
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Membrane Proteins / physiology
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Models, Molecular
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Protein Conformation
Substances
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AP1M2 protein, human
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AP3M2 protein, human
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Adaptor Protein Complex 1
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Adaptor Protein Complex 2
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Adaptor Protein Complex 3
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Adaptor Protein Complex alpha Subunits
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Adaptor Protein Complex mu Subunits
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Adaptor Proteins, Vesicular Transport
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Clathrin
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Membrane Proteins
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adaptor protein complex 1, mu 2 subunit
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adaptor protein complex 2, mu 2 subunit