Abstract
Methylation of the p16 gene was studied in 16 oesophageal tumours. Five (31%) of the tumours were found to be methylated in exon 1 and eight (50%) were methylated in exon 2. The loss of p16 protein correlated with methylation of exon 1 (P = 0.005). However, methylation of exon 2, but not exon 1, was found to be associated with late stage tumours (P = 0.01). We conclude that the methylation of exon 2 of p16 may have effects on the progression of oesophageal tumours that are independent of the expression of the p16 protein.
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Aged
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Aged, 80 and over
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology
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Cyclin-Dependent Kinase Inhibitor p16 / genetics
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Cyclin-Dependent Kinase Inhibitor p16 / metabolism*
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DNA Methylation*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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Esophageal Neoplasms / genetics
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Esophageal Neoplasms / metabolism*
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Esophageal Neoplasms / pathology
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Exons*
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Female
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Humans
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Immunohistochemistry
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Male
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Middle Aged
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Neoplasm Staging
Substances
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Cyclin-Dependent Kinase Inhibitor p16
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DNA, Neoplasm