Analysis of BRCA1 involvement in breast cancer in Indian women

J Biosci. 2000 Mar;25(1):19-23. doi: 10.1007/BF02985177.

Abstract

The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular patho-genesis of breast cancer and was in accordance with its well-documented tumour suppressive function.

MeSH terms

  • Alleles
  • BRCA1 Protein / analysis*
  • Breast Neoplasms / genetics*
  • Chromosomes, Human, Pair 17
  • Female
  • Humans
  • India
  • Loss of Heterozygosity
  • Neoplastic Syndromes, Hereditary / genetics*
  • Polymerase Chain Reaction

Substances

  • BRCA1 Protein