Immunohistochemical analysis of expression of a 65 kDa oncofetal protein (p65), epidermal growth factor receptor (EGFR), oncogene c-erb B2 and tumor suppressor gene p53 protein products in breast cancer patients

H Niewiadomska; M Mirowski; M Stempień; J Z Błoński; W Czyz; J Switalska; E Matyga; M Hanausek; R Wierzbicki

Immunohistochemical analysis of expression of a 65 kDa oncofetal protein (p65), epidermal growth factor receptor (EGFR), oncogene c-erb B2 and tumor suppressor gene p53 protein products in breast cancer patients

Neoplasma. 2000;47(1):8-14.

Authors

H Niewiadomska¹, M Mirowski, M Stempień, J Z Błoński, W Czyz, J Switalska, E Matyga, M Hanausek, R Wierzbicki

Affiliation

¹ Department of Oncology, Medical University, Lodz, Poland.

PMID: 10870681

Abstract

Paraffin-embedded tissue slides from 88 infiltrating ductal breast carcinoma were examined by immunohistochemistry technique with the use of monoclonal antibody against human p65 antigen and polyclonal antibody against p65-like protein present in fetal bovine serum. Immunohistochemical analysis of expression of growth factor receptors (EGFR), protein product of oncogene c-erb B2 as well as protein product of mutated anti-oncogene p53 was also done. It was established that there is no correlation between p65 and c-erbB2, EGFR or p53 expression. In low differentiated tumors (grade III) high p53 index and high EGFR and c-erbB2 expression was connected with low p65 expression. The lack of c-erbB2 and EGFR and low p53 expression was combined usually with high p65 oncoprotein levels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Breast Neoplasms / diagnosis
Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Carcinoma, Intraductal, Noninfiltrating / diagnosis
Carcinoma, Intraductal, Noninfiltrating / genetics
Carcinoma, Intraductal, Noninfiltrating / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
ErbB Receptors / genetics
ErbB Receptors / metabolism*
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Prognosis
Receptor, ErbB-2 / genetics
Receptor, ErbB-2 / metabolism*
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism*

Substances

Antibodies, Monoclonal
Biomarkers, Tumor
Carrier Proteins
HSAJ2425 protein, human
Intracellular Signaling Peptides and Proteins
Neoplasm Proteins
Tumor Suppressor Protein p53
ErbB Receptors
Receptor, ErbB-2